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Helicobacter pylori proinflammatory protein up-regulates NF-κB as a cell-translocating Ser/Thr kinase : Helicobacter pylori proinflammatory protein up-regulates NF-kappa B as a cell-translocating Ser/Thr kinase

Cited 45 time in Web of Science Cited 47 time in Scopus

Kim, Do Jin; Park, Kang-Seo; Kim, Jung-Ho; Yang, Sang-Hwa; Yoon, Ji Young; Han, Byeong-Gu; Kim, Hyoun Sook; Lee, Sang Jae; Jang, Jun Young; Kim, Kyoung Hoon; Kim, Mi Jung; Song, Jin-Su; Kim, Hie-Joon; Park, Chung-Mo; Lee, Sang-Kyou; Lee, Byung Il; Suh, Se Won

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National Academy of Sciences
Proceedings of the National Academy of Sciences of the United States of America, Vol.107 No.50, pp.21418-21423
There has been considerable interest in virulence genes in the plasticity region of Helicobacter pylori, but little is known about many of these genes. JHP940, one of the virulence factors encoded by the plasticity region of H. pylori strain J99, is a proinflammatory protein that induces tumor necrosis factor-alpha and interleukin-8 secretion as well as enhanced translocation of NF-kappa B in cultured macrophages. Here we have characterized the structure and function of JHP940 to provide the framework for better understanding its role in inflammation by H. pylori. Our work demonstrates that JHP940 is the first example of a eukaryotic-type Ser/Thr kinase from H. pylori. We show that JHP940 is catalytically active as a protein kinase and translocates into cultured human cells. Furthermore, the kinase activity is indispensable for indirectly up-regulating phosphorylation of NF-kappa B p65 at Ser276. Our results, taken together, contribute significantly to understanding the molecular basis of the role of JHP940 in inflammation and subsequent pathogenesis caused by H. pylori. We propose to rename the jhp940 gene as ctkA (cell translocating kinase A).
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