Browse

Helicobacter pylori proinflammatory protein up-regulates NF-κB as a cell-translocating Ser/Thr kinase

Cited 36 time in Web of Science Cited 39 time in Scopus
Authors
Kim, Do Jin; Park, Kang-Seo; Kim, Jung-Ho; Yang, Sang-Hwa; Yoon, Ji Young; Han, Byeong-Gu; Kim, Hyoun Sook; Lee, Sang Jae; Jang, Jun Young; Kim, Kyoung Hoon; Kim, Mi Jung; Song, Jin-Su; Kim, Hie-Joon; Park, Chung-Mo; Lee, Sang-Kyou; Lee, Byung Il; Suh, Se Won
Issue Date
2010-12
Citation
Proceedings of the National Academy of Sciences of the United States of America, Vol.107 No.50, pp.21418-21423
Abstract
There has been considerable interest in virulence genes in the plasticity region of Helicobacter pylori, but little is known about many of these genes. JHP940, one of the virulence factors encoded by the plasticity region of H. pylori strain J99, is a proinflammatory protein that induces tumor necrosis factor-alpha and interleukin-8 secretion as well as enhanced translocation of NF-kappa B in cultured macrophages. Here we have characterized the structure and function of JHP940 to provide the framework for better understanding its role in inflammation by H. pylori. Our work demonstrates that JHP940 is the first example of a eukaryotic-type Ser/Thr kinase from H. pylori. We show that JHP940 is catalytically active as a protein kinase and translocates into cultured human cells. Furthermore, the kinase activity is indispensable for indirectly up-regulating phosphorylation of NF-kappa B p65 at Ser276. Our results, taken together, contribute significantly to understanding the molecular basis of the role of JHP940 in inflammation and subsequent pathogenesis caused by H. pylori. We propose to rename the jhp940 gene as ctkA (cell translocating kinase A).
ISSN
0027-8424
URI
https://hdl.handle.net/10371/172094
DOI
https://doi.org/10.1073/pnas.1010153107
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse