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TERT mRNA expression is up-regulated in MCF-7 cells and a mouse mammary organ culture (MMOC) system by endosulfan treatment

Cited 8 time in Web of Science Cited 7 time in Scopus
Authors
Je, Kang Hoon; Kim, Ki-Nam; Nam, Kyung-Woo; Cho, Myung-Haing; Mar, Woongchon
Issue Date
2005-03
Citation
Archives of Pharmacal Research, Vol.28 No.3, pp.351-357
Keywords
endosulfantelomerasemouse mammary organ culture (MMOC)
Abstract
Endosulfan is one of the organochlorine pesticides, which are well-known endocrine disruptors (EDs), and it acts as an estrogen agonist. Estrogen is a group of-hormones that play an important role in mammary gland function and are implicated in mammary carcinogenesis. In the present study, we studied the effects of endosulfan on nodule like alveolar lesion (NLAL) formation in mouse mammary gland development using a mouse mammary gland organ culture (MMOC) system. Although endosulfan-treated mammary glands did not form NLALs, more alveolar buds were formed in this group than in the negative control (vehicle-treated) group. In addition, telomerase reverse transcriptase (TERT) mRNA expression levels were increased in enclosulfan-treated mammary glands in a dose-dependent manner. Telomerase can be activated by estrogen, therefore, we examined the effects of endosulfan on telomerase activity, and found that the telomerase activity in estrogen receptor-positive MCF-7 cells was up-regulated by endosulfan treatment. Moreover, this activation was accompanied by the up-regulation of the TERT mRNA expression. Also, transient expression assays using CAT reporter plasmids containing various fragments of the TERT promoter showed that this imperfect palindromic estrogen-responsive element is almost certainly responsible for the transcriptional activation by endosulfan. These results may help elucidate the endocrine disrupting mechanism of endosulfan.
ISSN
0253-6269
URI
https://hdl.handle.net/10371/172301
DOI
https://doi.org/10.1007/BF02977804
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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