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Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester)

Cited 13 time in Web of Science Cited 14 time in Scopus
Authors
Minai-Tehrani, Arash; Jiang, Hu-Lin; Kim, You-Kyoung; Chung, Youn-Sun; Yu, Kyeong-Nam; Kim, Ji-Eun; Shin, Ji-Young; Hong, Seong-Ho; Lee, Jae-Ho; Kim, Hye-Joon; Chang, Seung-Hee; Park, Sungjin; Kang, Bit Na; Cho, Chong-Su; Cho, Myung-Haing
Issue Date
2012-07
Citation
International Journal of Pharmaceutics, Vol.431 No.1-2, pp.197-203
Keywords
Cancer gene therapyOsteopontinsiRNAPoly(amino ester)
Abstract
Gene therapy using small interfering RNA (siRNA) is a novel strategy for effective anticancer therapies. However, low gene transfection efficiency and technical difficulties linked to siRNA delivery limit their practical application for gene delivery. Therefore, development of effective siRNA carriers is required. Overexpression of osteopontin (OPN) and its association with tumorigenesis has been reported in a majority of breast cancers. In this study, we used siRNA against OPN (siOPN) and investigated the possible OPN-dependent signaling pathway and the potential use of poly(amino ester) (PAE) based on glycerol propoxylate triacrylate (GPT) and spermine (SPE) for siRNA delivery. The GPT-SPE could effectively condense siRNA and protect the siRNA from RNaseA enzyme degradation. GPT-SPE/siRNA complexes showed good intracellular uptake and high gene silencing efficiency in vitro. Furthermore, in the breast cancer xenograft model, intratumoral injection of GPT-SPE/siOPN significantly inhibited tumor growth. These results demonstrated that silencing of OPN effectively suppressed the growth of breast cancer cells and further suggested that delivery of siRNA using GPT-SPE may act as an effective gene carrier for cancer therapy. (C) 2012 Elsevier B.V. All rights reserved.
ISSN
0378-5173
URI
https://hdl.handle.net/10371/172400
DOI
https://doi.org/10.1016/j.ijpharm.2012.04.028
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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