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Detection of methylation damage in DNA of gastric cancer tissues using P-32-postlabelling assay

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Authors
Kim, Dae-Yong; Cho, Myung-Haing; Yang, Han-Kwang; Hemminki, Kari; Kim, Jin-Pok; Jang, Ja-June; Kumar, Rajiv
Issue Date
1999-10
Citation
Japanese Journal of Cancer Research, Vol.90 No.10, pp.1104-1108
Keywords
gastric cancerDNA adductP-32-postlabellingDNA damage
Abstract
Gastric cancer is the most common cancer in Korea. The causes are still unknown but it has been speculated that gastric cancer is associated with consumption of foods rich in nitrates/nitrites or a high dietary intake of salt or pickled food. In the present study, we studied the level of alkylated DNA adducts formed in gastric cancer tissues in comparison with that in normal gastric mucosa. DNA was extracted from surgically removed gastric cancer tissues and patient-matched normal gastric mucosa. The level of N-7-methyldeoxyguanosine was measured by P-32-postlabelling assay after high performance liquid chromatography (HPLC) enrichment. We found that the level of N-7-methyldeoxyguanosine of gastric cancerous tissues was significantly higher than that of normal gastric mucosa (P=0.01685).
ISSN
0910-5050
URI
https://hdl.handle.net/10371/172408
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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