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Lentivirus-AIMP2-DX2 shRNA Suppresses Cell Proliferation by Regulating Akt1 Signaling Pathway in the Lungs of AIMP2(+/-) Mice

Cited 7 time in Web of Science Cited 9 time in Scopus
Authors
Hwang, Soon-Kyung; Chang, Seung-Hee; Minai-Tehrani, Arash; Kim, Yeon-Soo; Cho, Myung-Haing
Issue Date
2013-06
Citation
Journal of Aerosol Medicine and Pulmonary Drug Delivery, Vol.26 No.3, pp.165-173
Keywords
aerosol gene deliveryshRNA AIMP2-DX2angiogenesisAkt1 signaling pathway
Abstract
Background: The long-term survival of lung cancer patients treated with conventional therapies remains poor and has changed little in decades. The need for novel approaches remains urgent. Aerosol-mediated delivery of genes has potential for the treatment of a broad spectrum of pulmonary disorders and may offer numerous advantages over invasive modes of delivery. Methods: The potential effects of aerosol-delivered lentiviral-based short hairpin AIMP2 lacking exon 2 (shDX2) on lung tumorigenesis were studied. Lentiviral-based shDX2 was delivered into AIMP2(+/-) mice through a nose-only inhalation system twice a week for 4 weeks. Results and Conclusions: The effects of shDX2 on lung cancer progression and the Akt1-mTOR-p70S6K signaling pathway were evaluated. Long-term repeated delivery of lentiviral-based shDX2 suppressed lung tumor progression significantly by inhibiting Akt1-related signals and decreasing both protein synthesis and angiogenesis. In vivo, the aerosol-mediated application of lentiviral-based short hairpin RNAs was successful in achieving potent and specific knockdown of the target. The collective results indicate the therapeutic potential of the repeated delivery of shDX2 for lung cancer treatment and prevention.
ISSN
1941-2711
URI
https://hdl.handle.net/10371/172410
DOI
https://doi.org/10.1089/jamp.2011.0959
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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