Publications
Detailed Information
Suppression of tobacco carcinogen-induced lung tumorigenesis by aerosol-delivered glycerol propoxylate triacrylate-spermine copolymer/short hairpin Rab25 rna complexes in female A/J mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gankhuyag, Nomundelger | - |
dc.contributor.author | Yu, Kyeong Nam | - |
dc.contributor.author | Davaadamdin, Orkhonselenge | - |
dc.contributor.author | Lee, Somin | - |
dc.contributor.author | Cho, Won Young | - |
dc.contributor.author | Park, Changhoon | - |
dc.contributor.author | Jiang, Hu-Lin | - |
dc.contributor.author | Singh, Bijay | - |
dc.contributor.author | Chae, Chan-Hee | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.contributor.author | Cho, Chong-Su | - |
dc.date.accessioned | 2021-01-31T08:44:23Z | - |
dc.date.available | 2021-01-31T08:44:23Z | - |
dc.date.created | 2018-01-10 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.citation | Journal of Aerosol Medicine and Pulmonary Drug Delivery, Vol.30 No.2, pp.81-90 | - |
dc.identifier.issn | 1941-2711 | - |
dc.identifier.other | 9748 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172411 | - |
dc.description.abstract | Background: Rab25, a member of Rab family of small guanosine triphosphatase, is associated with progression of various types of human cancers, including lung cancer, the leading cause of cancer-associated deaths around the globe. Methods: In this study, we report the gene therapeutic effect of short hairpin Rab25 RNA (shRab25) on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung tumorigenesis in female A/J mice. Initially, mice (6 weeks old) were injected with single dose of NNK (2mg/0.1mL saline/mouse) by intraperitoneal injection to induce the tumor. Eight weeks later, shRab25 was complexed with glycerol propoxylate triacrylate-spermine (GPT-SPE) copolymer and delivered into tobacco-induced lung cancer models through a nose-only inhalation system twice a week for 2 months. Results: GPT-SPE/shRab25 largely decreased the tobacco-induced tumor numbers and tumor volume in the lungs compared to GPT-SPE- or GPT-SPE/shScr-delivered groups. Remarkably, aerosol-delivered GPT-SPE/shRab25 significantly decreased the expression level of Rab25 and other prominent apoptosis-related proteins in female A/J mice. The apoptosis in these mice was determined by detecting the expression level of Bcl-2, proliferating cell nuclear antigen, Bax, and further confirmed by TUNEL assay. Conclusions: Our results strongly confirm the tumorigenic role of Rab25 in tobacco carcinogen-induced lung cancer and hence demonstrate aerosol delivery of shRab25 as a therapeutic target for lung cancer treatment. | - |
dc.language | 영어 | - |
dc.publisher | Mary Ann Liebert Inc. | - |
dc.title | Suppression of tobacco carcinogen-induced lung tumorigenesis by aerosol-delivered glycerol propoxylate triacrylate-spermine copolymer/short hairpin Rab25 rna complexes in female A/J mice | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.1089/jamp.2016.1301 | - |
dc.citation.journaltitle | Journal of Aerosol Medicine and Pulmonary Drug Delivery | - |
dc.identifier.wosid | 000398812700001 | - |
dc.identifier.scopusid | 2-s2.0-85017173073 | - |
dc.citation.endpage | 90 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 81 | - |
dc.citation.volume | 30 | - |
dc.identifier.sci | 000398812700001 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Chae, Chan-Hee | - |
dc.contributor.affiliatedAuthor | Cho, Myung-Haing | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | GENE-THERAPY | - |
dc.subject.keywordPlus | CANCER PROGRESSION | - |
dc.subject.keywordPlus | CELL-PROLIFERATION | - |
dc.subject.keywordPlus | INHIBITS GROWTH | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | NNK | - |
dc.subject.keywordPlus | 4-(METHYLNITROSAMINO)-1-(3-PYRIDYL)-1-BUTANONE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ENDOCYTOSIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | aerosol-delivered shRab25 | - |
dc.subject.keywordAuthor | A | - |
dc.subject.keywordAuthor | J mice | - |
dc.subject.keywordAuthor | lung cancer | - |
dc.subject.keywordAuthor | NNK | - |
dc.subject.keywordAuthor | gene therapy | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.