Browse

Degradable Poly(ester amine) Based on Poly(ethylene glycol) Dimethacrylate and Polyethylenimine as a Gene Carrier

Cited 1 time in Web of Science Cited 1 time in Scopus
Authors
Yu, Jia-Hui; Huang, Jin; Nah, Jae-Woon; Cho, Myung-Haing; Cho, Chong-Su
Issue Date
2010-01
Citation
Journal of Applied Polymer Science, Vol.115 No.2, pp.1189-1198
Keywords
gene deliverydegradablecytotoxocitypoly(ester amine)polyethylenimine
Abstract
Degradable poly(ester amine) (PEA) based on poly(ethylene glycol) dimethacrylate (PMEG) and polyethylenimine (PEI) were synthesized by Michael addition reaction. The ratios of PEI to PMEG in PEAS were 0.99, 1.02, and 1.07 with corresponding number-average molecular weight of 1.3 x 10(4), 1.2 x 10(4), and 0.9 x 10(4), respectively. Degradation rate of PEA at pH 7.4 was higher than that at pH 5.6. Good plasmid condensation and protection ability was shown when N/P molar ratio of PEA to DNA was above 15 (N: nitrogen element in PEA, P: phosphate in DNA). PEA/DNA complexes had positive zeta potential, narrow size distribution, good dispersity, and spheric shape with size below 250 nm when N/P ratio was above 30, suggestion of their endocytosis potential. Compared with PEI 25 KDa, the PEAs showed essential nontoxic to HeLa, HepG2 and 293T cells. With an increase in the molecular weight of PMEG, the transfection efficiency of PEAS in HeLa, HepG2 and 293T showed a tendency to decrease as well as the percent decrease of gene transfection efficiency with serum. The mechanism of PEA-mediated gene transfection was attributed to "proton sponge effect" of PEI in the PEA. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 115: 1189-1198, 2010
ISSN
0021-8995
URI
https://hdl.handle.net/10371/172414
DOI
https://doi.org/10.1002/app.31234
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse