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Monocyclic carotenoid biosynthetic pathway in the yeast Phaffia rhodozyma (Xanthophyllomyces dendrorhous)

Cited 35 time in Web of Science Cited 42 time in Scopus
Authors
An, Gil-Hwan; Cho, Myung-Haing; Johnson, Eric A.
Issue Date
1999-08
Citation
Journal of Bioscience and Bioengineering, Vol.88 No.2, pp.189-193
Keywords
piperonyl butoxidemonocyclic carotenoidsPhaffia rhodozymaXanthophyllomyces dendrorhous
Abstract
The biosynthetic pathway of monocyclic carotenoids in the yeast Phaffia rhodozyma was studied by identifying carotenoids, applying inhibitors of carotenoid synthesis, and analyzing the carotenoids in carotenogenic mutants. Two carotenoids, torulene and 3,3'-dihydroxy-beta,phi-carotene-4,4'-dione (DCD), were identified from the yeast. Piperonyl butoxide inhibited dehydrogenation of carotenes and caused accumulation of neurosporene, lycopene, gamma-carotene, and beta-zeacarotene. Yellow mutants of P. rhodozyma produced mainly beta-carotene, which is a dicyclic carotene produced from neurosporene through lycopene. The yellow mutants grown with 0.01% triethylamine (TEA) or 2 mM 2-methylimidazole (MI) produced significantly increased amounts of torulene, which is a monocyclic carotene produced from neurosporene through beta-zeacarotene. When red strains of P. rhodozyma were grown with TEA or MI, they produced increased amounts of beta-zeacarotene, torulene, and 3-hydroxy-3',4'-didehydro-beta,phi-carotene-4-one (HDCO) (all monocyclic). DCD, accumulated especially in old cultures, was also increased in MI or TEA cultures coincident with the increases in HDCO. We propose the following monocyclic carotenoid pathway in P. rhodozyma: neurosporene-->beta-zeacarotene-->gamma-carotene-->torulene-->HDCO-->DCD.
ISSN
1389-1723
URI
https://hdl.handle.net/10371/172421
DOI
https://doi.org/10.1016/S1389-1723(99)80200-X
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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