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Galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine as a gene carrier for hepatocyte-targeting

Cited 133 time in Web of Science Cited 144 time in Scopus
Authors
Jiang, Hu-Lin; Kwon, Jung-Taek; Kim, Eun-Mi; Kim, You-Kyoung; Arote, Rohidas; Jere, Dhananjay; Jeong, Hwan-Jeong; Jang, Mi-Kyeong; Nah, Jae-Woon; Xu, Cheng-Xiong; Park, In-Kyu; Cho, Myung-Haing; Cho, Chong-Su
Issue Date
2008-10
Citation
Journal of Controlled Release, Vol.131 No.2, pp.150-157
Keywords
Gene therapyTargeting gene deliveryGalactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimineCytotoxicityTransfection efficiencyIntravenous administration
Abstract
Chitosan and chitosan derivatives have been proposed as alternative and biocompatible cationic polymers for non-viral gene delivery. However. the low transfection efficiency and low specificity of chitosan is an aspect of this approach that must be addressed prior to any clinical applications. In the present study a chitosan derivative, galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine (Gal-PEG-CHI-g-PEI), was investigated as a potential hepatocyte-targeting gene carrier. The composition of Gal-PEG-CHI-g-PEI was characterized using H-1 nuclear magnetic resonance (H-1 NMR), and the particle size and zeta potential of Gal-PEG-CHI-g-PEI/DNA complexes were measured using dynamic light scattering (DLS). The Gal-PEG-CHI-g-PEI exhibited lower cytotoxicity compared to PEI 25K as a control. Likewise, Gal-PEC-CHI-g-PEI/DNA complexes showed good hepatocyte specificity. Furthermore, Gal-PEG-CHI-g-PEI/DNA complexes transfected liver cells more effectively than PEI 25K in vivo after intravenous (i.v.) administration. Together, these results suggest that Gal-PEG-CHI-g-PEI, which has improved transfection efficiency and hepatocyte specificity both in vitro and in vivo, may be useful for gene therapy. (C) 2008 Elsevier B.V. All rights reserved.
ISSN
0168-3659
URI
https://hdl.handle.net/10371/172425
DOI
https://doi.org/10.1016/j.jconrel.2008.07.029
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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