S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Journal Papers (저널논문_수의학과)
Galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine as a gene carrier for hepatocyte-targeting
- Jiang, Hu-Lin; Kwon, Jung-Taek; Kim, Eun-Mi; Kim, You-Kyoung; Arote, Rohidas; Jere, Dhananjay; Jeong, Hwan-Jeong; Jang, Mi-Kyeong; Nah, Jae-Woon; Xu, Cheng-Xiong; Park, In-Kyu; Cho, Myung-Haing; Cho, Chong-Su
- Issue Date
- Journal of Controlled Release, Vol.131 No.2, pp.150-157
- Gene therapy; Targeting gene delivery; Galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine; Cytotoxicity; Transfection efficiency; Intravenous administration
- Chitosan and chitosan derivatives have been proposed as alternative and biocompatible cationic polymers for non-viral gene delivery. However. the low transfection efficiency and low specificity of chitosan is an aspect of this approach that must be addressed prior to any clinical applications. In the present study a chitosan derivative, galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine (Gal-PEG-CHI-g-PEI), was investigated as a potential hepatocyte-targeting gene carrier. The composition of Gal-PEG-CHI-g-PEI was characterized using H-1 nuclear magnetic resonance (H-1 NMR), and the particle size and zeta potential of Gal-PEG-CHI-g-PEI/DNA complexes were measured using dynamic light scattering (DLS). The Gal-PEG-CHI-g-PEI exhibited lower cytotoxicity compared to PEI 25K as a control. Likewise, Gal-PEC-CHI-g-PEI/DNA complexes showed good hepatocyte specificity. Furthermore, Gal-PEG-CHI-g-PEI/DNA complexes transfected liver cells more effectively than PEI 25K in vivo after intravenous (i.v.) administration. Together, these results suggest that Gal-PEG-CHI-g-PEI, which has improved transfection efficiency and hepatocyte specificity both in vitro and in vivo, may be useful for gene therapy. (C) 2008 Elsevier B.V. All rights reserved.
- Files in This Item: There are no files associated with this item.