Browse

In Situ Single Cell Monitoring by Isocyanide-Functionalized Ag and Au Nanoprobe-Based Raman Spectroscopy

Cited 8 time in Web of Science Cited 7 time in Scopus
Authors
Lee, So Yeong; Jang, Soo Hwa; Cho, Myung-Haing; Kim, Young Min; Cho, Keun Chang; Ryu, Pan Dong; Gong, Myoung-Seon; Joo, Sang-Woo
Issue Date
2009-09
Citation
Journal of Microbiology and Biotechnology, Vol.19 No.9, pp.904-910
Keywords
In situ cellular imagingnanoparticlessurface modificationRaman spectroscopycell monitoring
Abstract
The development of effective cellular imaging requires a specific labeling method for targeting, tracking, and monitoring cellular/molecular events in the living organism. For this purpose, we studied the cellular uptake of isocyanide-functionalized silver and gold nanoparticles by surface-enhanced Raman scattering (SERS). Inside a single mammalian cell, we could monitor the intracellular behavior of such nanoparticles by measuring the SERS spectra. The NC stretching band appeared clearly at similar to 2,100 cm(-1) in the well-isolated spectral region from many organic constituents between 300 and 1,700 or 2,800 and 3,600 cm(-1). The SERS marker band at similar to 2,100 cm(-1) could be used to judge the location of the isocyanide-functionalized nanoparticles inside the cell without much spectral interference from other cellular constituents. Our results demonstrate that isocyanide-modified silver or gold nanoparticle-based SERS may have high potential for monitoring and imaging the biological processes at the single cell level.
ISSN
1017-7825
URI
https://hdl.handle.net/10371/172431
DOI
https://doi.org/10.4014/jmb.0901.009
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse