S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Journal Papers (저널논문_수의학과)
In Situ Single Cell Monitoring by Isocyanide-Functionalized Ag and Au Nanoprobe-Based Raman Spectroscopy
- Lee, So Yeong; Jang, Soo Hwa; Cho, Myung-Haing; Kim, Young Min; Cho, Keun Chang; Ryu, Pan Dong; Gong, Myoung-Seon; Joo, Sang-Woo
- Issue Date
- Journal of Microbiology and Biotechnology, Vol.19 No.9, pp.904-910
- In situ cellular imaging; nanoparticles; surface modification; Raman spectroscopy; cell monitoring
- The development of effective cellular imaging requires a specific labeling method for targeting, tracking, and monitoring cellular/molecular events in the living organism. For this purpose, we studied the cellular uptake of isocyanide-functionalized silver and gold nanoparticles by surface-enhanced Raman scattering (SERS). Inside a single mammalian cell, we could monitor the intracellular behavior of such nanoparticles by measuring the SERS spectra. The NC stretching band appeared clearly at similar to 2,100 cm(-1) in the well-isolated spectral region from many organic constituents between 300 and 1,700 or 2,800 and 3,600 cm(-1). The SERS marker band at similar to 2,100 cm(-1) could be used to judge the location of the isocyanide-functionalized nanoparticles inside the cell without much spectral interference from other cellular constituents. Our results demonstrate that isocyanide-modified silver or gold nanoparticle-based SERS may have high potential for monitoring and imaging the biological processes at the single cell level.
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