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Receptor-mediated gene delivery into antigen presenting cells using mannosylated chitosan/DNA nanoparticles

Cited 88 time in Web of Science Cited 94 time in Scopus
Authors
Kim, Tae Hee; Nah, Jae Woon; Cho, Myung-Haing; Park, Tae Gwan; Cho, Chong Su
Issue Date
2006-09
Citation
Journal of Nanoscience and Nanotechnology, Vol.6 No.9-10, pp.2796-2803
Keywords
receptor-mediated gene deliverychitosanmannoseantigen presenting cellsnanoparticle
Abstract
Dendritic cells (DCs) are professional antigen presenting cells that induce, sustain, and regulate immune responses. Gene modification of DCs is of particular interest for immunotherapy of diseases where the immunes system has failed or is abnormally regulated, such as in cancer or autoimmune disease. Gene transfer using non-viral vectors is a promising approach for the safe delivery of therapeutic DNA. Among various non-viral vectors, chitosan is considered to be a good candidate for gene delivery system, however, lack of cell specificity and low transfection of chitosan need to be overcome prior to clinical use. In this study, mannosylated chitosan (MC) was prepared to induce the receptor-mediated endocytosis and targeting into antigen presenting cells (APCs), especially DCs having mannose receptors. MC showed great ability to form complexes with DNA and showed suitable physicochemical properties for gene delivery system. It had low cytotoxicity and exhibited much enhanced gene transfer efficiency on the macrophage cell line than chitosan itself. Also, MC/DNA complex was more efficient for transferring IL-12 gene into DCs rather than water-soluble chitosan (WSC)/DNA one, which resulted in better induction of INF-gamma from DCs. Therefore, MC is a promising gene delivery system for repeated administration to maintain sustained gene expression, thereby opening the possibility for immunotherapy.
ISSN
1533-4880
URI
https://hdl.handle.net/10371/172438
DOI
https://doi.org/10.1166/jnn.2006.434
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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