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Beclin1-induced Autophagy Abrogates Radioresistance of Lung Cancer Cells by Suppressing Osteopontin

Cited 31 time in Web of Science Cited 35 time in Scopus
Authors
Chang, Seung-Hee; Minai-Tehrani, Arash; Shin, Ji-Young; Park, Sungjin; Kim, Ji-Eun; Yu, Kyeong-Nam; Hong, Seong-Ho; Hong, Choong-Man; Lee, Kee-Ho; Beck, George R., Jr.; Cho, Myung-Haing
Issue Date
2012-05
Citation
Journal of Radiation Research, Vol.53 No.3, pp.422-432
Keywords
Osteopontin(OPN)Beclin-1(BECN1)Gamma-irradiationp53Angiogenesis
Abstract
Osteopontin (OPN) serves as an indicator of resistance to radiotherapy. However, the role of OPN in the development of acquired radioresistance in human lung cancer cells has not yet been fully elucidated. Therefore, the potential importance of OPN as a marker of lung cancer with a potential significant role in the development of radioresistance against repeated radiotherapy has prompted us to define the pathways by which OPN regulates lung cancer cell growth. In addition, autophagy has been reported to play a key role in the radiosensitization of cancer cells. Here, we report that increased OPN expression through induction of nuclear p53 following irradiation was inhibited by exogenous beclin-1 (BECN1). Our results clearly show that BECN1 gene expression led to induction of autophagy and inhibition of cancer cell growth and angiogenesis. Our results suggest that the induction of autophagy abrogated the radioresistance of the cancer cells. Interestingly, we showed that knockdown of OPN by lentivirus-mediated shRNA induced the autophagy of human lung cancer cell. Taken together, these results suggest that OPN and BECN1 can be molecular targets for overcoming radioresistance by controlling autophagy.
ISSN
0449-3060
URI
https://hdl.handle.net/10371/172445
DOI
https://doi.org/10.1269/jrr.11148
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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