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Aerosol delivery of kinase-deficient Akt1 attenuates Clara cell injury induced by naphthalene in the lungs of dual luciferase mice

Cited 7 time in Web of Science Cited 8 time in Scopus
Authors
Minai-Tehrani, Arash; Park, Young-Chan; Hwang, Soon-Kyung; Kwon, Jung-Taek; Chang, Seung-Hee; Park, Sung-Jin; Yu, Kyeong-Nam; Kim, Ji-Eun; Shin, Ji-Young; Kim, Ji-Hye; Kang, Bitna; Hong, Seong-Ho; Cho, Myung-Haing
Issue Date
2011-12
Citation
Journal of Veterinary Science, Vol.12 No.4, pp.309-317
Keywords
aerosol deliveryAkt1Clara cellERKluciferase activitynaphthalene
Abstract
Conventional lung cancer therapies are associated with poor survival rates; therefore, new approaches such as gene therapy are required for treating cancer. Gene therapies for treating lung cancer patients can involve several approaches. Among these, aerosol gene delivery is a potentially more effective approach. In this study, Akt1 kinase-deficient (KD) and wild-type (WT) Akt1 were delivered to the lungs of CMV-LucR-cMyc-IRES-LucF dual reporter mice through a nose only inhalation system using glucosylated polyethylenimine and naphthalene was administrated to the mice via intraperitoneal injection. Aerosol delivery of Akt1 WT and naphthalene treatment increased protein levels of downstream substrates of Akt signaling pathway while aerosol delivery of Akt1 KD did not. Our results showed that naphthalene affected extracellular signal-regulated kinase (ERK) protein levels, ERK-related signaling, and induced Clara cell injury. However, Clara cell injury induced by naphthalene was considerably attenuated in mice exposed to Aka KD. Furthermore, a dual luciferase activity assay showed that aerosol delivery of Akt1 WT and naphthalene treatment enhanced cap-dependent protein translation, while reduced cap-dependent protein translation was observed after delivering Aka KD. These studies demonstrated that our aerosol delivery is compatible for in vivo gene delivery.
ISSN
1229-845X
URI
https://hdl.handle.net/10371/172461
DOI
https://doi.org/10.4142/jvs.2011.12.4.309
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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