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Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hyuk-Mi | - |
dc.contributor.author | Song, Sung-Ok | - |
dc.contributor.author | Cha, Sang-Ho | - |
dc.contributor.author | Wee, Sung-Bok | - |
dc.contributor.author | Bischoff, Karyn | - |
dc.contributor.author | Park, Sung-Won | - |
dc.contributor.author | Son, Seong-Wan | - |
dc.contributor.author | Kang, Hwan-Goo | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.date.accessioned | 2021-01-31T08:47:36Z | - |
dc.date.available | 2021-01-31T08:47:36Z | - |
dc.date.created | 2020-12-10 | - |
dc.date.issued | 2013-06 | - |
dc.identifier.citation | Journal of Veterinary Science, Vol.14 No.2, pp.143-150 | - |
dc.identifier.issn | 1229-845X | - |
dc.identifier.other | 118958 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172462 | - |
dc.description.abstract | Monoclonal antibody (mAb, NVRQS-DON) against deoxynivalenol (DON) was prepared. DON-Ag coated enzyme linked immunosorbent assay (ELISA) and DON-Ab coated ELISA were prepared by coating the DON-BSA and DON mAb. Quantitative DON calculation ranged from 50 to 4,000 ng/mL for DON-Ab coated ELISA and from 25 to 500 ng/mL for DON-Ag coated ELISA. 50% of inhibitory concentration values of DON, HT-2, 15-acetyl-DON, and nivalenol were 23.44, 22,545, 5,518 and 5,976 ng/mL based on the DON-Ab coated ELISA. Cross-reactivity levels of the mAb to HT-2, 15-acetyl-DON, and nivalenol were 0.1, 0.42, and 0.40 %. The intra- and interassay precision coefficient variation (CV) were both < 10 %. In the mAb-coated ELISA, mean DON recovery rates in animal feed (0 to 1,000 mu g/kg) ranged from 68.34 to 95.49% (CV; 4.10 to 13.38%). DON in a buffer solution (250, 500 and 1,000 ng/mL) was isolated using 300 mu g of NVRQS-DON and 3 mg of magnetic nanoparticles (MNPs). The mean recovery rates of DON using this mAb-MNP system were 75.2, 96.9, and 88.1% in a buffer solution spiked with DON (250, 500, and 1,000 ng/mL). Conclusively we developed competitive ELISAs for detecting DON in animal feed and created a new tool for DON extraction using mAb-coupled MNPs. | - |
dc.language | 영어 | - |
dc.publisher | 대한수의학회 | - |
dc.title | Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.4142/jvs.2013.14.2.143 | - |
dc.citation.journaltitle | Journal of Veterinary Science | - |
dc.identifier.wosid | 000321025200005 | - |
dc.identifier.scopusid | 2-s2.0-84880102297 | - |
dc.citation.endpage | 150 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 143 | - |
dc.citation.volume | 14 | - |
dc.identifier.sci | 000321025200005 | - |
dc.identifier.kciid | ART001775981 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Cho, Myung-Haing | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | IMMUNOCHROMATOGRAPHIC STRIP | - |
dc.subject.keywordPlus | MYCOTOXIN DEOXYNIVALENOL | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | BEAD SEPARATION | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | FRAGMENT | - |
dc.subject.keywordPlus | AFFINITY | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordAuthor | deoxynivalenol, ELISA, magnetic nanoparticles, monoclonal antibody | - |
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