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Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay

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dc.contributor.authorLee, Hyuk-Mi-
dc.contributor.authorSong, Sung-Ok-
dc.contributor.authorCha, Sang-Ho-
dc.contributor.authorWee, Sung-Bok-
dc.contributor.authorBischoff, Karyn-
dc.contributor.authorPark, Sung-Won-
dc.contributor.authorSon, Seong-Wan-
dc.contributor.authorKang, Hwan-Goo-
dc.contributor.authorCho, Myung-Haing-
dc.date.accessioned2021-01-31T08:47:36Z-
dc.date.available2021-01-31T08:47:36Z-
dc.date.issued2013-06-
dc.identifier.citationJournal of Veterinary Science, Vol.14 No.2, pp.143-150-
dc.identifier.issn1229-845X-
dc.identifier.other118958-
dc.identifier.urihttps://hdl.handle.net/10371/172462-
dc.description.abstractMonoclonal antibody (mAb, NVRQS-DON) against deoxynivalenol (DON) was prepared. DON-Ag coated enzyme linked immunosorbent assay (ELISA) and DON-Ab coated ELISA were prepared by coating the DON-BSA and DON mAb. Quantitative DON calculation ranged from 50 to 4,000 ng/mL for DON-Ab coated ELISA and from 25 to 500 ng/mL for DON-Ag coated ELISA. 50% of inhibitory concentration values of DON, HT-2, 15-acetyl-DON, and nivalenol were 23.44, 22,545, 5,518 and 5,976 ng/mL based on the DON-Ab coated ELISA. Cross-reactivity levels of the mAb to HT-2, 15-acetyl-DON, and nivalenol were 0.1, 0.42, and 0.40 %. The intra- and interassay precision coefficient variation (CV) were both < 10 %. In the mAb-coated ELISA, mean DON recovery rates in animal feed (0 to 1,000 mu g/kg) ranged from 68.34 to 95.49% (CV; 4.10 to 13.38%). DON in a buffer solution (250, 500 and 1,000 ng/mL) was isolated using 300 mu g of NVRQS-DON and 3 mg of magnetic nanoparticles (MNPs). The mean recovery rates of DON using this mAb-MNP system were 75.2, 96.9, and 88.1% in a buffer solution spiked with DON (250, 500, and 1,000 ng/mL). Conclusively we developed competitive ELISAs for detecting DON in animal feed and created a new tool for DON extraction using mAb-coupled MNPs.-
dc.subjectdeoxynivalenol, ELISA, magnetic nanoparticles, monoclonal antibody-
dc.titleDevelopment of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay-
dc.typeArticle-
dc.contributor.AlternativeAuthor조명행-
dc.identifier.doi10.4142/jvs.2013.14.2.143-
dc.citation.journaltitleJournal of Veterinary Science-
dc.identifier.scopusid2-s2.0-84880102297-
dc.citation.endpage150-
dc.citation.number2-
dc.citation.startpage143-
dc.citation.volume14-
dc.identifier.rimsid118958-
dc.identifier.sci000321025200005-
dc.identifier.kciidART001775981-
dc.contributor.affiliatedAuthorCho, Myung-Haing-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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