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Cell cycle was disturbed in the MNNG-induced initiation stage during in vitro two-stage transformation of Balb/3T3 cells
DC Field | Value | Language |
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dc.contributor.author | Fang, MZ | - |
dc.contributor.author | Mar, WC | - |
dc.contributor.author | Cho, MYUNG HAING | - |
dc.date.accessioned | 2021-01-31T08:50:32Z | - |
dc.date.available | 2021-01-31T08:50:32Z | - |
dc.date.created | 2017-11-17 | - |
dc.date.issued | 2001-06 | - |
dc.identifier.citation | Toxicology, Vol.163 No.2-3, pp.175-184 | - |
dc.identifier.issn | 0300-483X | - |
dc.identifier.other | 6455 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172515 | - |
dc.description.abstract | In vitro two-stage transformation. an important method for the screening of carcinogens, is a valuable approach For the mechanistic study of multi-stage carcinogenesis. However, very little is known about the molecular and cellular mechanisms, particularly in terms of cell cycle control during in vitro two-stage transformation. We improved the in vitro two-stage transformation method using N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) as an initiator and cadmium as a promoter. and reconfirmed the promotional effect of cadmium (Fang et al., 2001a). To determine the alterations of cell cycle control in the MNNG-induced initiation stage during transformation, we examined the effects of MNNG on Balb/3T3 A31 cell growth, and determined the alterations of the protein and/or mRNA levels of cyclins B1, D1, E, and C, PCNA, GADD45, p27, and wild-type p53. After 4 hour treatment of MNNG. populations of G2/M phase distribution and apoptotic fraction and the cyclin G mRNA level increased, while the cyclin B1 mRNA Level decreased in a concentration-dependent manner. Wild-type p53. p27, and GADD45 protein levels also increased as a function of MNNG concentrations. However, cyclin D1, cyclin E, and PCNA expressions remained unchanged. During the initiation stage, PCNA protein expression decreased on the first day after MNNG-treatment, then increased gradually during rhs Following 6 days; and further increased on the first day after cadmium treatment. Although wild-type p53 and p27 protein expressions also showed temporary retardation on the first day after MNNG-treatment, the expressions increased gradually during the following 6 days, but decreased rapidly by the cadmium treatment. These results indicated that during the initiation stage, MNNG induced G2/M arrest and apoptosis with increased expressions of wild-type p53, p27, and GADD45 proteins: and down-regulated mRNA level of cyclin B1 and up-regulated mRNA level of cyclin G. In addition, although a few of the G2/M-arrested cells proliferated gradually. most cells continued to be suppressed and inactivated by the over-expressions of wild-type p53 and p27 until the cadmium treatment. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Cell cycle was disturbed in the MNNG-induced initiation stage during in vitro two-stage transformation of Balb/3T3 cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.1016/S0300-483X(01)00400-0 | - |
dc.citation.journaltitle | Toxicology | - |
dc.identifier.wosid | 000169812800012 | - |
dc.identifier.scopusid | 2-s2.0-0035927922 | - |
dc.citation.endpage | 184 | - |
dc.citation.number | 2-3 | - |
dc.citation.startpage | 175 | - |
dc.citation.volume | 163 | - |
dc.identifier.sci | 000169812800012 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Mar, WC | - |
dc.contributor.affiliatedAuthor | Cho, MYUNG HAING | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CHEMICAL CARCINOGENS | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | GROWTH ARREST | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordPlus | N-METHYL-N&apos | - |
dc.subject.keywordPlus | -NITRO-N-NITROSOGUANIDINE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CHECKPOINT | - |
dc.subject.keywordPlus | G2/M | - |
dc.subject.keywordPlus | G(2) | - |
dc.subject.keywordAuthor | cell cycle | - |
dc.subject.keywordAuthor | carcinogens | - |
dc.subject.keywordAuthor | MNNG-treatment | - |
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