Browse

Chitosan nanoparticles show rapid extrapulmonary tissue distribution and excretion with mild pulmonary inflammation to mice

Cited 22 time in Web of Science Cited 24 time in Scopus
Authors
Choi, Mina; Cho, Minjung; Han, Beom Seok; Hong, Jin; Jeong, Jayoung; Park, Sangjin; Cho, Myung-Haing; Kim, Kwangmeyung; Cho, Wan-Seob
Issue Date
2010-11
Citation
Toxicology Letters, Vol.199 No.2, pp.144-152
Keywords
Hydrophobically modified glycol chitosanPulmonary delivery vehicleIntratracheal instillationTissue distributionElimination
Abstract
Pulmonary delivery of nanoparticles (NP) conjugated with therapeutic agents has been considered recently for both lung disorders and systemic circulation. Hydrophobically modified glycol chitosan (HGC) NP have previously shown excellent deposition to the tumor site and non-destructive intracellular release. Here, we evaluated the kinetics and toxicity of HGC NP by intratracheal instillation to mice. HGC NP showed a positive charge and average hydrodynamic size was around 350 nm. The half-life of NP in the lung was determined as 131.97+/-50.51 h. NP showed rapid uptake into systemic circulation and excretion via urine which was peaked at 6 h after instillation. Although HGC NP were distributed to several extrapulmonary organs, the levels were extremely low and transient. HGC NP induced transient neutrophilic pulmonary inflammation from 6 h to day 3 after instillation. Expression of pro-inflammatory cytokines (IL-1 beta, IL-6, and TNF-alpha) and chemokine (MIP-1 alpha) in lung showed an increase from 1 h to 24 h after instillation and recovered thereafter. Our findings suggest that HGC NP can be successful candidates for use as pulmonary delivery vehicles, owing to their excellent biocompatibility, transiency, and low pulmonary toxicity, and property of rapid elimination without accumulation. Crown Copyright (C) 2010 Published by Elsevier Ireland Ltd. All rights reserved.
ISSN
0378-4274
URI
https://hdl.handle.net/10371/172525
DOI
https://doi.org/10.1016/j.toxlet.2010.08.016
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse