Browse

The peptidyl prolyl isomerase, PIN1 induces angiogenesis through direct interaction with HIF-2α

Cited 1 time in Web of Science Cited 1 time in Scopus
Authors
Choi, Min-A; Saeidi, Soma; Han, Hyeong-jun; Kim, Su-Jung; Kwon, Nayoung; Kim, Do-Hee; Min, Sang-Hyun; Choi, Bu Young; Surh, Young-Joon
Issue Date
2020-01
Citation
Biochemical and Biophysical Research Communications, p. In Press
Keywords
AngiogenesisHIF-2αHypoxiaPeptidyl prolyl isomerasePIN1VEGF
Abstract
© 2020 Elsevier Inc.PIN1, the peptidyl-prolyl isomerase (PPIase), is an enzyme that changes the conformation of phosphoproteins. The conformational change induced by PIN1 alters the function and stability of the target proteins. PIN1 is overexpressed in many different types of malignancies, including breast, lung, cervical, brain and colorectal tumors. PIN1 overexpression has been associated with activation of multiple oncogenic signaling pathways during tumor development. Hypoxia-inducible factor 2α (HIF-2α), a transcription factor activated in hypoxia, plays a role in erythropoiesis, glycolysis, tissue invasion, metastasis and angiogenesis. In this study, we found the direct interaction between HIF-2α and PIN1 in colorectal cancer HCT116 cells. Notably, serine 16 and lysine 63 residues of PIN1 were critical for its interaction with HIF-2α. When PIN1 protein was silenced by transient transfection of PIN1 short interfering RNA, the expression of HIF-2α was attenuated under a hypoxic condition. Moreover, genetic and pharmacologic inhibition of PIN1 abrogated the expression of vascular endothelial growth factor and angiogenesis. The cycloheximide chase experiment revealed the stabilization of HIF-2α by PIN1. Both WW and PPIase domains of PIN1 appear to be critical for its interaction with HIF-2α.
ISSN
0006-291X
URI
https://hdl.handle.net/10371/172559
DOI
https://doi.org/10.1016/j.bbrc.2020.08.015
Files in This Item:
There are no files associated with this item.
Appears in Collections:
Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Journal Papers (저널논문_분자의학 및 바이오제약학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse