Publications
Detailed Information
17-Oxo-docosahexaenoic acid induces Nrf2-mediated expression of heme oxygenase-1 in mouse skin in vivo and in cultured murine epidermal cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jamil, Muhammad Usman | - |
dc.contributor.author | Kim, Jimin | - |
dc.contributor.author | Yum, Hye-Won | - |
dc.contributor.author | Kim, Seong Hoon | - |
dc.contributor.author | Kim, Su-Jung | - |
dc.contributor.author | Kim, Do-Hee | - |
dc.contributor.author | Cho, Nam-Chul | - |
dc.contributor.author | Na, Hye-Kyung | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.date.accessioned | 2021-01-31T09:19:19Z | - |
dc.date.available | 2021-01-31T09:19:19Z | - |
dc.date.created | 2020-04-06 | - |
dc.date.created | 2020-04-06 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.citation | Archives of Biochemistry and Biophysics, Vol.679, p. 108156 | - |
dc.identifier.issn | 0003-9861 | - |
dc.identifier.other | 94849 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172569 | - |
dc.description.abstract | Recently, growing attention has been given to new classes of bioactive lipid mediators derived from omega-3 polyunsaturated fatty acids, such as docosahexaenoic acid (DHA), especially in the context of their role as endogenous signal modulators. One such molecule is 17-oxo-DHA, generated from DHA by the action of COX2 and a dehydrogenase. The redox-sensitive transcription factor, Nrf2 plays a key role in cellular stress responses. In the present study, the effects of 17-oxo-DHA on Nrf2-mediated expression of cytoprotective enzymes were examined in mouse skin in vivo and cultured murine epidermal JB6 cells. Topical application of 17-oxo-DHA markedly elevated the nuclear localization of Nrf2 and expression of heme oxygenase-1 (HO-1) and NAD(P) H:quinone oxidoreductase-1 in hairless mouse skin. In contrast to 17-oxo-DHA, the non-electrophilic metabolic precursor 17-hydroxy-DHA was a much weaker inducer of Nrf2 activation and its target protein expression. Likewise, 17-oxo-DHA significantly enhanced nuclear translocation and transcriptional activity of Nrf2 with concomitant upregulation of HO-1 expression in cultured JB6 cells. 17-Oxo-DHA was a much stronger inducer of Nrf2-mediated antioxidant response than its parent molecule, DHA. HO-1 expression was abolished in Nrf2 knockdown JB6 cells or embryo fibroblasts from Nrf2 knock out mice. 17-Oxo-DHA also markedly reduced the level of Keap1 protein by inducing ubiquitination. Mutation of Cys151 and Cys273 in Keap1 abrogated 17-oxo-DHA-induced ubiquitination and proteasome-mediated degradation of Keap1 as well as HO-1 expression, suggesting that these cysteine residues are putative sites for 17-oxo-DHA binding. Further, Keap1 degradation stimulated by 17-oxo-DHA coincided with accumulation of the autophagy substrate, p62/SQSTM1. | - |
dc.language | 영어 | - |
dc.publisher | Academic Press | - |
dc.title | 17-Oxo-docosahexaenoic acid induces Nrf2-mediated expression of heme oxygenase-1 in mouse skin in vivo and in cultured murine epidermal cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 서영준 | - |
dc.identifier.doi | 10.1016/j.abb.2019.108156 | - |
dc.citation.journaltitle | Archives of Biochemistry and Biophysics | - |
dc.identifier.wosid | 000525443900001 | - |
dc.identifier.scopusid | 2-s2.0-85076201276 | - |
dc.citation.startpage | 108156 | - |
dc.citation.volume | 679 | - |
dc.identifier.sci | 000525443900001 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Surh, Young-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | POLYUNSATURATED FATTY-ACIDS | - |
dc.subject.keywordPlus | SUBSTRATE ADAPTER PROTEIN | - |
dc.subject.keywordPlus | CUL3-BASED E3 LIGASE | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | EICOSAPENTAENOIC ACID | - |
dc.subject.keywordPlus | KEAP1 | - |
dc.subject.keywordPlus | NRF2 | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | OMEGA-3-FATTY-ACIDS | - |
dc.subject.keywordPlus | UBIQUITINATION | - |
dc.subject.keywordAuthor | Docosahexaenoic acid | - |
dc.subject.keywordAuthor | Heme oxygenase-1 | - |
dc.subject.keywordAuthor | Mouse skin | - |
dc.subject.keywordAuthor | Nrf2 | - |
dc.subject.keywordAuthor | Omega-3 polyunsaturated fatty acids | - |
dc.subject.keywordAuthor | 17-Oxo-DHA | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.