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Inhibition of phorbol ester-induced COX-2 expression by some edible African plants

Cited 28 time in Web of Science Cited 39 time in Scopus
Authors
Na, Hye‐Kyung; Mossanda, Kensese S.; Lee, Ji‐Yoon; Surh, Young‐Joon
Issue Date
2004
Citation
BioFactors, Vol.21 No.1-4, pp.149-153
Keywords
African medicinal plantscyclooxygenase-2NF-kappa BTPAMCF10A cellsmouse skin carcinogenesis
Abstract
Cancer bush (CB, Sutherlandia frutescens), Devil's claw (DEV, Harpagophytum procumbens), Rooibos tea (RT, Aspalathus linearis), and Bambara groundnut (BB, Vignea subterranean) have been used to treat some malignancies and inflammatory disorders in Africa. However, biochemical basis for chemopreventive effects of these medicinal plants remains unclear. An abnormally elevated expression of cyclooxygenase-2 (COX-2) has been implicated in pathogenesis and progression of carcinogenesis. In the present study, we found that the methanol extracts of CB, DEV, RT, and BB inhibited, to a different extent, 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced COX-2 expression in human breast epithelial (MCF10A) cells and in mouse skin in vivo. To determine the molecular mechanism of COX-2 inhibition by the above medicinal plants, we examined their effects on activation of NF-kappaB which is one of the major transcription factors responsible for regulating COX-2 expression. Methanol extracts of both CB and BB inhibited the DNA binding of NF-kappaB activated by TPA in MCF10A cells in a dose-dependent manner. Based on above findings, CB and BB are likely to inhibit TPA-induced COX-2 expression through suppression of DNA binding of NF-kappaB, which may contribute to the chemopreventive or chemoprotective activity of these African plants.
ISSN
0951-6433
URI
https://hdl.handle.net/10371/172630
DOI
https://doi.org/10.1002/biof.552210130
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Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Journal Papers (저널논문_분자의학 및 바이오제약학과)
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