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Potentiation of etoposide-induced apoptosis in HeLa cells by co-treatment with KG-135, a quality-controlled standardized ginsenoside formulation

Cited 12 time in Web of Science Cited 14 time in Scopus
Authors
Lee, Won-Hee; Choi, Joon-Seok; Kim, Hyun Young; Park, Jeong-Hill; Park, Byoung Duck; Cho, Seung Ju; Lee, Seung-Ki; Surh, Young-Joon
Issue Date
2010-08
Citation
Cancer Letters, Vol.294 No.1, pp.74-81
Keywords
EtoposideGinsengKG-135Apoptosisp53
Abstract
Our previous studies demonstrated that KG-135, a quality-controlled red ginseng-specific formulation containing approximately equal amounts of three major ginsenosides (Rk1, Rg3 and Rg5), down-regulated Cl cyclin-dependent kinase in HeLa cells. In the present work, we have found that KG-135 potentates cytotoxicity of etoposide by modulating apoptotic signaling. Co-treatment of etoposide and KG-135 markedly elevated the expression and phosphorylation at the serine 15 residue of p53 as well as the cellular levels of Bax and p21(Waf1/Cip1). The increased accumulation and phosphorylation of p53 (Ser15) were attenuated by treatment of cells with wortmannin, a pan-phosphatidylinosito1-3 kinase inhibitor. Moreover, co-treatment of etoposide and KG-135 enhanced mitochondrial localization of Bax. Our results indicate that etoposide-induced apoptosis in HeLa cells can be potentiated in the presence of KG-135 through a mechanism that involves the stabilization of p53 and the stimulation of Bax- and p21-mediated apoptotic signaling pathways. These findings suggest that KG-135 represents a useful candidate adjuvant for the treatment of cancers that could potentially minimize the adverse effects of current clinical chemotherapeutics. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
ISSN
0304-3835
URI
https://hdl.handle.net/10371/172638
DOI
https://doi.org/10.1016/j.canlet.2010.01.024
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Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Journal Papers (저널논문_분자의학 및 바이오제약학과)
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