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Capsaicin induced apoptosis of B16-F10 melanoma cells through down-regulation of Bcl-2

Cited 46 time in Web of Science Cited 51 time in Scopus
Authors
Jun, Hye-Seung; Park, Taesun; Lee, Chang Ki; Kang, Mi Kyung; Park, Mi Sun; Il Kang, Ho; Surh, Young-Joon; Kim, Ok Hee
Issue Date
2007-05
Citation
Food and Chemical Toxicology, Vol.45 No.5, pp.708-715
Keywords
capsaicinapoptosisB16-F10 melanoma cellsDNA fragmentationflow cytometryBcl-2
Abstract
Capsaicin (8-methyl-N-vanillyl-6-nonenamide), a pungent ingredient of hot chili peppers, has been reported to possess substantial anticarcinogenic and antimutagenic activities. In the present study, we investigated the effect of capsaicin on induction of apoptosis in highly metastatic B16-F10 murine melanoma cells. Capsaicin inhibited growth of B16-F10 cells in a concentration-dependent manner. Proapoptotic effect of capsaicin was evidenced by nuclear condensation, internucleosomal DNA fragmentation, in situ terminal nick-end labeling of fragmented DNA (TUNEL), and an increased sub G1 fraction. Treatment of B16-F10 cells with capsaicin caused release of mitochondrial cytochrome c, activation of caspase-3, and cleavage of poly (ADP-ribose) polymerase in a dose-dependent manner. Furthermore, Bcl-2 expression in the B16-F10 cells was slightly down-regulated by capsaicin treatment. In contrast, there were no alterations in the levels of Bax in capsaicin-treated cells. Collectively, these findings indicate that capsaicin-induces apoptosis of B16-F10 melanoma cells via down-regulation the Bcl-2. (c) 2006 Elsevier Ltd. All rights reserved.
ISSN
0278-6915
URI
https://hdl.handle.net/10371/172703
DOI
https://doi.org/10.1016/j.fct.2006.10.011
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Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Journal Papers (저널논문_분자의학 및 바이오제약학과)
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