S-Space Graduate School of Convergence Science and Technology (융합과학기술대학원) Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과) Journal Papers (저널논문_분자의학 및 바이오제약학과)
Molecular mechanism underlying anti-apoptotic and anti-inflammatory effects of Mamao (Antidesma thwaitesianum Mull. Arg.) polyphenolics in human breast epithelial cells
- Puangpronpitag, Darunee; Yongvanit, Puangrat; Boonsiri, Patcharee; Suttajit, Maitree; Areejitranusorn, Premjai; Na, Hye-Kyung; Surh, Young-Joon
- Issue Date
- Food Chemistry, Vol.127 No.4, pp.1450-1458
- A. thwaitesianum Mull. Arg.; Apoptosis; Inflammation; MCF10A cells; Mitogen-activated protein kinases
- There has been increasing interest in finding natural antioxidants to prevent free radical damage and retard the progress of chronic inflammatory diseases. Our previous data demonstrated the strong antioxidant properties of polyphenolics in Mamao seed (MS) and Mamao marc (MM) extracts. In this study we further investigated the effect of MS and MM polyphenolics on hydrogen peroxide (H(2)O(2))-induced apoptosis and tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation, using human breast epithelial (MCF10A) cells. MS and MM extracts conferred dose-dependent protection against H(2)O(2)-induced apoptosis by inhibiting PARP/caspase-3 cleavage, inducing anti-apoptotic Bcl-2 expression, and down-regulating pro-apoptotic Bax. Moreover, MS and MM polyphenolics inhibited TPA-induced COX-2 and NF-kappa B activation by blocking the degradation of cytoplasmic I kappa B alpha, as well as subsequent nuclear translocation of p65 and attenuation of the activation of ERK, but not JNK and p38. These data establish the molecular mechanism for the anti-apoptotic and anti-inflammatory effects of MS and MM polyphenolics. (C) 2011 Elsevier Ltd. All rights reserved.
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