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Protective effect of resveratrol on β-amyloid-induced oxidative PC12 cell death

Cited 306 time in Web of Science Cited 344 time in Scopus
Authors
Jang, Jung-Hee; Surh, Young-Joon
Issue Date
2003-04
Citation
Free Radical Biology and Medicine, Vol.34 No.8, pp.1100-1110
Keywords
beta-amyloidapoptosisNF-kappa Boxidative stressPC12 cellsreactive oxygen intermediatesresveratrolfree radicals
Abstract
Beta-amyloid peptide is considered to be responsible for the formation of senile plaques that accumulate in the brains of patients with Alzheimer's disease. There has been compelling evidence supporting the idea that beta-amyloid-induced cytotoxicity is mediated through the generation of reactive oxygen intermediates (ROIs). Considerable attention has been focused on identifying phytochemicals that are able to scavenge excess ROIs, thereby protecting against oxidative stress and cell death. Resveratrol (3,5,4'-trihydroxy-trans-stilbene), a phytoalexin found in the skin of grapes, has strong antioxidative properties that have been associated with the protective effects of red wine consumption against coronary heart disease ("the French paradox"). In this study, we have investigated the effects of resveratrol on beta-amyloid-induced oxidative cell death in cultured rat pheochromocytoma (PC12) cells. PC12 cells treated with beta-amyloid exhibited increased accumulation of intracellular ROI and underwent apoptotic death as determined by characteristic morphological alterations and positive in situ terminal end-labeling (TUNEL staining). Beta-amyloid treatment also led to the decreased mitochondrial membrane potential, the cleavage of poly(ADPribose)polymerase, an increase in the Bax/Bcl-X-L ratio, and activation of c-Jun N-terminal kinase. Resveratrol attenuated beta-amyloid-induced cytotoxicity, apoptotic features, and intracellular ROI accumulation. Beta-amyloid transiently induced activation of NF-kappaB in PC12 cells, which was suppressed by resveratrol pretreatment. (C) 2003 Elsevier Inc.
ISSN
0891-5849
URI
https://hdl.handle.net/10371/172726
DOI
https://doi.org/10.1016/S0891-5849(03)00062-5
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Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Journal Papers (저널논문_분자의학 및 바이오제약학과)
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