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Jaceosidin induces apoptosis in ras-transformed human breast epithelial cells through generation of reactive oxygen species
DC Field | Value | Language |
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dc.contributor.author | Kim, Min-Jung | - |
dc.contributor.author | Kim, Do-Hee | - |
dc.contributor.author | Lee, Ki Won | - |
dc.contributor.author | Yoon, Do-Young | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.date.accessioned | 2021-01-31T10:15:13Z | - |
dc.date.available | 2021-01-31T10:15:13Z | - |
dc.date.created | 2017-11-15 | - |
dc.date.issued | 2007-03 | - |
dc.identifier.citation | Annals of the New York Academy of Sciences, Vol.1095, pp.483-495 | - |
dc.identifier.issn | 0077-8923 | - |
dc.identifier.other | 3589 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172760 | - |
dc.description.abstract | Extracts of Artemisia plants possess anti-inflammatory and antioxidative activities. Eupatilin (5,7-dihydroxy-3',4',6-tri-methoxynavone), a pharmacologically active flavone derived from Artemisia asiatica, was shown to inhibit phorbol ester-induced cyclooxygenase-2 expression and NF-kappa B activation in mouse skin, and also to induce cell cycle arrest in ras-transformed human mammary epithelial (MCF10A-ras) cells. In this article, we examined the ability of jaceosidin (4',5,7-trihydroxy-3',6-dimethoxyflavone) isolated from Artemisia argyi to inhibit the proliferation of MCF10A-ras cells. Jaceosidin reduced the viability of MCF10A-ras cells to a greater extent than eupatilin. Jaceosidin treatment resulted in increased intracellular accumulation of reactive oxygen species (ROS) in MCF10A-ras cells, which was blocked by the antioxidant N-acetylcysteine (NAC). NAC attenuated jaceosidin-induced cytotoxicity. To better assess the proapoptotic effects of jaceosidin, we analyzed the treated cells by the flow cytometry. MCF10A-ras cells treated with jaceosidin (100 mu M) exhibited the increased proportion of hypodiploid or apoptotic cells (48.72% as composed to 7.78% in control cells). Jaceosidin treatment also increased the ratio of proapoptotic Bax to the antiapoptotic Bcl-2 and induced the cleavage of caspase-3 and poly(ADP-ribose)polymerase (PARP). Moreover, jaceosidin elevated the expression of p53 and p21, while the compound inhibited the activation of ERK1/2 that is an important component of cell survival signaling. | - |
dc.language | 영어 | - |
dc.publisher | New York Academy of Sciences | - |
dc.title | Jaceosidin induces apoptosis in ras-transformed human breast epithelial cells through generation of reactive oxygen species | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 서영준 | - |
dc.identifier.doi | 10.1196/annals.1397.052 | - |
dc.citation.journaltitle | Annals of the New York Academy of Sciences | - |
dc.identifier.wosid | 000245947400051 | - |
dc.identifier.scopusid | 2-s2.0-34247853280 | - |
dc.citation.endpage | 495 | - |
dc.citation.startpage | 483 | - |
dc.citation.volume | 1095 | - |
dc.identifier.sci | 000245947400051 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Surh, Young-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | ARTEMISIA PLANTS | - |
dc.subject.keywordPlus | MAP KINASES | - |
dc.subject.keywordPlus | CANCER-PREVENTION | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | PROTEIN-KINASES | - |
dc.subject.keywordPlus | C-JUN | - |
dc.subject.keywordPlus | EUPATILIN | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | JNK | - |
dc.subject.keywordAuthor | jaceosidin | - |
dc.subject.keywordAuthor | eupatilin | - |
dc.subject.keywordAuthor | MCF10A-ras cells | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | ERK | - |
dc.subject.keywordAuthor | Akt | - |
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