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Piceatannol inhibits phorbol ester-induced expression of COX-2 and iNOS in HR-1 hairless mouse skin by blocking the activation of NF-kappa B and AP-1

Cited 19 time in Web of Science Cited 20 time in Scopus
Authors
Liu, Lijia; Li, Jianchun; Kundu, Joydeb Kumar; Surh, Young-Joon
Issue Date
2014-12
Citation
Inflammation Research, Vol.63 No.12, pp.1013-1021
Keywords
PiceatannolCyclooxygenase-2Inducible nitric oxide synthaseNuclear factor-kappaBActivator protein-1Mouse skin
Abstract
The present study was aimed at elucidating the molecular mechanisms of anti-inflammatory activity of piceatannol (trans-3,4,3',5'-tetrahydroxystilbene) in mouse skin in vivo. Female HR-1 hairless mice were topically treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) with or without piceatannol pretreatment. Epidermal protein expression was assessed by Western blot analysis. The cyclooxygenase-2 (COX-2) expression was detected by immunohistochemistry. The DNA binding of nuclear factor-kappaB (NF-kappa B) and activator protein-1 (AP-1) was examined by the electrophoretic mobility gel shift assay. The catalytic activity of I kappa B alpha kinase-beta (IKK beta) was measured by in vitro kinase assay. Pretreatment with piceatannol attenuated TPA-induced expression of COX-2 and inducible nitric oxide synthase (iNOS) in mouse skin. Piceatannol diminished nuclear translocation and the DNA binding of NF-kappa B through the blockade of phosphorylation and subsequent degradation of I kappa B alpha. Piceatannol attenuated the catalytic activity of IKK beta and inhibited the phosphorylation of mitogen-activated protein (MAP) kinases in TPA-treated mouse skin. In addition, piceatannol decreased TPA-induced expression of c-Fos and the DNA binding of AP-1. Piceatannol inhibits TPA-induced COX-2 and iNOS expression by blocking the activation of NF-kappa B and AP-1 via suppression of the IKK beta activity and phosphorylation of MAP kinases, which provides a mechanistic basis of its anti-inflammatory effects in mouse skin.
ISSN
1023-3830
URI
https://hdl.handle.net/10371/172769
DOI
https://doi.org/10.1007/s00011-014-0777-6
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Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Journal Papers (저널논문_분자의학 및 바이오제약학과)
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