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Effects of dried longan seed (Euphoria longana Lam.) extract on VEGF secretion and expression in colon cancer cells and angiogenesis in human umbilical vein endothelial cells

Cited 11 time in Web of Science Cited 12 time in Scopus
Authors
Panyathep, Atita; Chewonarin, Teera; Taneyhill, Khanittha; Surh, Young-Joon; Vinitketkumnuen, Usanee
Issue Date
2013-07
Citation
Journal of Functional Foods, Vol.5 No.3, pp.1088-1096
Keywords
Dried longan seedFractionVEGFSW480 cellAngiogenesisHUVEC
Abstract
Angiogenesis is a critical event in cancer metastasis, via delivery of needed oxygen and nutrients to tumor cells. Anti-angiogenesis is one strategy for controlling cancer progression. We herein report anti-angiogenesis activity of dried longan seeds using colon adenocarcinoma cells (SW480 cells) and human umbilical vein endothelial cells (HUVECs). Sephadex LH-20 column chromatography was used for separate three dried longan seed fractions. We firstly evaluated vascular endothelial cell growth factor (VEGF) secretion, expression and colony formation of SW480 cells, using enzyme-linked immunosorbent assay (ELISA), Western blot analysis and soft agar assays. Meanwhile cell proliferation, gelatinase activity and tube formation of HUVECs were determined via proliferation assay, gelatin zymography and in vitro tube formation assay, respectively. The results suggest that dried longan seed fractions could be potential angiogenic inhibitors not only interruption of VEGF secretion and expression in SW480 cells but also abrogation of cell proliferation, the activity of gelatinase and tube formation of HUVECs. (C) 2013 Elsevier Ltd. All rights reserved.
ISSN
1756-4646
URI
https://hdl.handle.net/10371/172787
DOI
https://doi.org/10.1016/j.jff.2013.03.004
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Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Journal Papers (저널논문_분자의학 및 바이오제약학과)
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