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15-Deoxy-Δ 12,14 -prostaglandin J 2 induces COX-2 expression through Akt-driven AP-1 activation in human breast cancer cells: a potential role of ROS : 15-Deoxy-delta(12,14)-prostaglandin J(2) induces COX-2 expression through Akt-driven AP-1 activation in human breast cancer cells: a potential role of ROS
Cited 63 time in
Web of Science
Cited 63 time in Scopus
- Authors
- Issue Date
- 2008-04
- Publisher
- Oxford University Press
- Citation
- Carcinogenesis, Vol.29 No.4, pp.688-695
- Abstract
- Recent studies suggest that inflammation is causally linked to carcinogenesis. Cyclooxygenase-2 (COX-2), a rate-limiting enzyme in the biosynthesis of prostaglandins, is inappropriately expressed in various cancers and hence recognized as one of the hallmarks of chronic inflammation-associated malignancies. However, the mechanistic role of COX-2 as a link between inflammation and cancer remains undefined. Here, we report that 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), one of the final products of COX-mediated arachidonic acid metabolism, upregulates the expression of COX-2 in the human breast cancer MCF-7 cell line. 15d-PGJ(2)-induced COX-2 expression was mediated by activation of Akt and subsequently activator protein-1 (AP-1). Furthermore, 15d-PGJ(2) formed reactive oxygen species, which led to increased phosphorylation of Akt, DNA binding of AP-1 and expression of COX-2. In contrast to 15d-PGJ(2), 9,10-dihydro-15d-PGJ(2) did not elicit any of effects induced by 15d-PGJ(2) in this study, suggesting that an electrophilic carbon center present in 15d-PGJ(2) is critical for COX-2 expression as well activation of upstream signal transduction induced by this cyclopentenone prostaglandin. Taken together, these observations suggest that 15d-PGJ(2) produced by COX-2 overexpression may function as a positive regulator of COX-2 in human breast cancer MCF-7 cells.
- ISSN
- 0143-3334
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