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Genistein inhibits phorbol ester-induced NF-kappa B transcriptional activity and COX-2 expression by blocking the phosphorylation of p65/RelA in human mammary epithelial cells
DC Field | Value | Language |
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dc.contributor.author | Chung, Myung-Hoon | - |
dc.contributor.author | Kim, Do-Hee | - |
dc.contributor.author | Na, Hye-Kyung | - |
dc.contributor.author | Kim, Jung-Hwan | - |
dc.contributor.author | Kim, Ha-Na | - |
dc.contributor.author | Haegeman, Guy | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.date.accessioned | 2021-01-31T10:20:19Z | - |
dc.date.available | 2021-01-31T10:20:19Z | - |
dc.date.created | 2017-11-15 | - |
dc.date.issued | 2014-10 | - |
dc.identifier.citation | Mutation Research, Vol.768, pp.74-83 | - |
dc.identifier.issn | 0027-5107 | - |
dc.identifier.other | 1201 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172839 | - |
dc.description.abstract | Genistein, an isoflavone present in soy products, has chemopreventive effects on mammary carcinogenesis. In the present study, we have investigated the effects of genistein on phorbol ester-induced expression of cyclooxygenase-2 (COX-2) that plays an important role in the pathophysiology of inflammation-associated carcinogenesis. Pretreatment of cultured human breast epithelial (MCF10A) cells with genistein reduced COX-2 expression induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). There are multiple lines of evidence supporting that the induction of COX-2 is regulated by the eukaryotic transcription factor NF-kappa B. Genistein failed to inhibit TPA-induced nuclear translocation and DNA binding of NF-kappa B as well as degradation of I kappa B. However, genistein abrogated the TPA-induced transcriptional activity of NF-kappa B as determined by the luciferase reporter gene assay. Genistein inhibited phosphorylation of the p65 subunit of NF-kappa B and its interaction with cAMP regulatory element-binding protein-binding protein (CBP)/p300 and TATA-binding protein (TBP). TPA-induced NF-kappa B phosphorylation was abolished by pharmacological inhibition of extracellular signal-regulated kinase (ERK). Likewise, pharmacologic inhibition or dominant negative mutation of ERK suppressed phosphorylation of p65. The above findings, taken together, suggest that genistein inhibits TPA-induced COX-2 expression in MCF10A cells by blocking ERK-mediated phosphorylation of p65 and its subsequent interaction with CBP and TBP. (C) 2014 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Genistein inhibits phorbol ester-induced NF-kappa B transcriptional activity and COX-2 expression by blocking the phosphorylation of p65/RelA in human mammary epithelial cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 서영준 | - |
dc.identifier.doi | 10.1016/j.mrfmmm.2014.04.003 | - |
dc.citation.journaltitle | Mutation Research | - |
dc.identifier.wosid | 000343844400009 | - |
dc.identifier.scopusid | 2-s2.0-84929048857 | - |
dc.citation.endpage | 83 | - |
dc.citation.startpage | 74 | - |
dc.citation.volume | 768 | - |
dc.identifier.sci | 000343844400009 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Surh, Young-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | TUMOR-NECROSIS-FACTOR | - |
dc.subject.keywordPlus | TATA-BINDING PROTEIN | - |
dc.subject.keywordPlus | P65 SUBUNIT | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 EXPRESSION | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | TRANSACTIVATION DOMAIN | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | REGULATED KINASE | - |
dc.subject.keywordPlus | JAPANESE MEN | - |
dc.subject.keywordAuthor | Chemoprevention | - |
dc.subject.keywordAuthor | Genistein | - |
dc.subject.keywordAuthor | Cyclooxygenase-2 | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | MCF10A cells | - |
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