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Nitric oxide induces expression of cyclooxygenase-2 in mouse skin through activation of NF-kappa B
DC Field | Value | Language |
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dc.contributor.author | Chun, Kyung-Soo | - |
dc.contributor.author | Cha, Hyun-Ho | - |
dc.contributor.author | Shin, Jun-Wan | - |
dc.contributor.author | Na, Hye-Kyung | - |
dc.contributor.author | Park, Kwang-Kyun | - |
dc.contributor.author | Chung, Won-Yoon | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.date.accessioned | 2021-01-31T10:20:26Z | - |
dc.date.available | 2021-01-31T10:20:26Z | - |
dc.date.created | 2017-11-15 | - |
dc.date.issued | 2004-03 | - |
dc.identifier.citation | Carcinogenesis, Vol.25 No.3, pp.445-454 | - |
dc.identifier.issn | 0143-3334 | - |
dc.identifier.other | 4252 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172841 | - |
dc.description.abstract | Inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) are frequently overexpressed in tumor tissues or transformed cells. In the present work, we assessed the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on expression of iNOS and COX-2 in mouse skin. Topical application to the dorsal skin of female ICR mice of 10 nmol TPA led to maximal induction of iNOS and COX-2 protein expression at similar to2 and 4 h, respectively. When applied topically onto shaven backs of mice 30 min prior to TPA, the NOS inhibitor aminoguanidine (AG) inhibited the expression of COX-2 protein at the pharmacologically effective dose. Pretreatment with a more specific iNOS inhibitor, N-G-nitro-l-arginine-methyl ester, also suppressed TPA-induced COX-2 expression. Immunohistochemical analysis of TPA-treated mouse skin using an anti-nitrotyrosine antibody reveals enhanced levels of nitrotyrosine protein localized in epidermal and dermal layers. Topical application of NO donors, such as sodium nitroprusside (SNP) and S-nitroso-N-acetyl-d,l-penicillamine, induced expression of COX-2 in mouse skin, which was attenuated by the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl imidazoline-1-oxyl 3-oxide. SNP treatment stimulated NF-kappaB activation in mouse skin, which was associated with the degradation of IkappaBalpha. Topical application of inhibitors of NF-kappaB, such as pyrrolidine dithiocarbamate or N-alpha-p-tosyl-l-lysine chloromethylketone, inhibited the SNP-induced COX-2 expression. SNP induced a weak but concentration-related increase in COX-2 expression in cultured mouse keratinocytes, which was abolished by treatment with SN50, a specific inhibitor of nuclear translocation of NF-kappaB. Mouse keratinocytes treated with SNP exhibited an elevated NF-kappaB-driven COX-2 promoter activity. Topical application of AG (10 mumol) prior to each TPA treatment after initiation reduced the multiplicity of papillomas by 44% at 22 weeks. In conclusion, up-regulation of COX-2 by NO may be mediated by activation of NF-kappaB in mouse skin, which provides a molecular mechanism by which COX-2 is induced during tumor promotion. | - |
dc.language | 영어 | - |
dc.publisher | Oxford University Press | - |
dc.title | Nitric oxide induces expression of cyclooxygenase-2 in mouse skin through activation of NF-kappa B | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 서영준 | - |
dc.identifier.doi | 10.1093/carcin/bgh021 | - |
dc.citation.journaltitle | Carcinogenesis | - |
dc.identifier.wosid | 000220058900020 | - |
dc.identifier.scopusid | 2-s2.0-1542399059 | - |
dc.citation.endpage | 454 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 445 | - |
dc.citation.volume | 25 | - |
dc.identifier.sci | 000220058900020 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Surh, Young-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PROSTAGLANDIN-ENDOPEROXIDE SYNTHASE-2 | - |
dc.subject.keywordPlus | ABERRANT CRYPT FOCI | - |
dc.subject.keywordPlus | TRANSGENIC MICE | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | S-NITROSYLATION | - |
dc.subject.keywordPlus | TUMOR PROMOTION | - |
dc.subject.keywordPlus | MESANGIAL CELLS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | E-2 SYNTHESIS | - |
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