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2-(Allylthio)pyrazine suppresses the growth and proliferation of human promyelocytic leukemia (HL-60) cells via induction of apoptosis

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Authors
Lee, E; Kong, G; Lee, SJ; Kim, ND; Surh, Young-Joon
Issue Date
1999-09
Citation
Anticancer Research, Vol.19 No.5B, pp.4073-4080
Keywords
2-(Allylthio)pyrazineapoptosishuman promyelocytic leukemia cellsorganosulfur compoundsantiproliferation
Abstract
Apoptosis or programmed cell death is a highly organized physiologic process of not only maintaining homeostasis but also selectively eliminating damaged or abnormal cells. Apoptotic destruction of predisposed cells may reduce the proportion of cells available for malignant progression. Thus, pharmacologic manipulation of apoptotic pathway is regarded as a novel strategy in cancer chemoprevention as well as therapy. 2-(Allylthio)pyrazine (2-AP), a pyrazine derivative of allylsulfide synthesized for use as a chemoprotective agent, has been shown to protect against experimental carcinogenesis and mutagenesis. The present study examined the capability of 2-AP to induce apoptosis in cultured human promyelocytic leukemia (HL-60) cells. Treatment of HL-60 cells with 2-AP led to suppression of viability and proliferation in a concentration-dependent manner: Microscopic examination of the treated cells revealed typical morphological features of apoptosis, such as nuclear fragmentation and chromatin condensation. Furthermore, cells treated with 2-AP exhibited internucleosomal DNA fragmentation. Flow cytometric analysis of HL-60 cells exposed to 2-AP showed appearance of a distinct peak representing the subdiploid cell population. 2-AP treatment dea eased the ratio of anti-apoptotic Bcl-2 to the death stimulating protein Bax, which may account for the molecular basis of apoptosis-inducing activity of this chemopreventive organosulfur derivative.
ISSN
0250-7005
URI
https://hdl.handle.net/10371/172902
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Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Journal Papers (저널논문_분자의학 및 바이오제약학과)
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