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15-Deoxy-Δ12,14-prostaglandin J2 Induces Epithelial-tomesenchymal Transition in Human Breast Cancer Cells and Promotes Fibroblast Activation : 15-Deoxy-Delta(12,14) prostaglandin J(2) Induces Epithelial-to-mesenchymal Transition in Human Breast Cancer Cells and Promotes Fibroblast Activation
DC Field | Value | Language |
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dc.contributor.author | Choi, Jeehye | - |
dc.contributor.author | Suh, Jin-Young | - |
dc.contributor.author | Kim, Do-Hee | - |
dc.contributor.author | Na, Hye-Kyung | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.date.accessioned | 2021-01-31T10:25:50Z | - |
dc.date.available | 2021-01-31T10:25:50Z | - |
dc.date.created | 2020-11-02 | - |
dc.date.issued | 2020-09 | - |
dc.identifier.citation | 대한암예방학회지, Vol.25 No.3, pp.152-163 | - |
dc.identifier.issn | 2288-3649 | - |
dc.identifier.other | 114467 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172922 | - |
dc.description.abstract | In inflammation-associated carcinogenesis, COX-2 is markedly overexpressed, resulting in accumulation of various prostaglandins. 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) is one of the terminal products of COX-2-catalyzed arachidonic acid catabolism with oncogenic potential. Epithelial-to-mesenchymal transition (EMT) is a process by which epithelial cells lose their polarity and adhesiveness, and thereby gain migratory and invasive properties. Treatment of human breast cancer MCF-7 cells with 15d-PGJ(2) induced EMT as evidenced by increased expression of Snail and ZEB1, with concurrent down-regulation of E-cadherin. Nuclear extract from 15d-PGJ(2) treated MCF-7 cells showed the binding of Snail and ZEB1 to E-box sequences present in the E-cadherin promoter, which accounts for repression of E-catherin expression. Unlike 15d-PGJ(2), its non-electrophilic analogue 9,10-dihydro-15d-PGJ(2) failed to induce EMT, suggesting that the alpha,beta-unsaturated carbonyl group located in the cyclopentenone ring of 15d-PGJ(2) is essential for its oncogenic function. Notably, the mRNA level of interleukin-8 (IL-8)/CXCL8 was highly elevated in 15d-PGJ(2)-stimulated MCF-7 cells. 15d-PGJ(2)-induced up-regulation of IL-8/CXCL8 expression was abrogated by silencing of Snail short interfering RNA. Treatment of normal fibroblast with conditioned medium obtained from cultures of MCF-7 cells undergoing EMT induced the expression of activated fibroblast marker proteins, alpha-smooth muscle actin and fibroblasts activation protein-alpha. Co-culture of normal fibroblasts with 15d-PGJ(2)-stimulated MCF-7 cells also activated normal fibroblast cells to cancer associated fibroblasts. Taken together, above findings suggest that 15d-PGJ(2) induces EMT through up-regulation of Snail expression and subsequent production of CXCL8 as a putative activator of fibroblasts, which may contribute to tumor-stroma interaction in inflammatory breast cancer microenvironment. | - |
dc.language | 영어 | - |
dc.publisher | 대한암예방학회 | - |
dc.title | 15-Deoxy-Δ12,14-prostaglandin J2 Induces Epithelial-tomesenchymal Transition in Human Breast Cancer Cells and Promotes Fibroblast Activation | - |
dc.title.alternative | 15-Deoxy-Delta(12,14) prostaglandin J(2) Induces Epithelial-to-mesenchymal Transition in Human Breast Cancer Cells and Promotes Fibroblast Activation | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 서영준 | - |
dc.identifier.doi | 10.15430/JCP.2020.25.3.152 | - |
dc.citation.journaltitle | 대한암예방학회지 | - |
dc.identifier.wosid | 000577160300003 | - |
dc.citation.endpage | 163 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 152 | - |
dc.citation.volume | 25 | - |
dc.identifier.sci | 000577160300003 | - |
dc.identifier.kciid | ART002629022 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Surh, Young-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 2 | - |
dc.subject.keywordPlus | E-CADHERIN | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 EXPRESSION | - |
dc.subject.keywordPlus | STROMAL FIBROBLASTS | - |
dc.subject.keywordPlus | TUMOR | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | SNAIL | - |
dc.subject.keywordPlus | MYOFIBROBLASTS | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | 15D-PGJ(2) | - |
dc.subject.keywordPlus | ZEB1 | - |
dc.subject.keywordAuthor | 15-Deoxy-Delta(12,14)-prostaglandin J(2) | - |
dc.subject.keywordAuthor | Breast cancer | - |
dc.subject.keywordAuthor | Cancer-associated fibroblasts | - |
dc.subject.keywordAuthor | Epithelial-to-mesenchymal transition | - |
dc.subject.keywordAuthor | Tumor microenvironment | - |
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