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Transforming growth factor-β induces apoptosis in activated murine T cells through the activation of caspase 1-1ike protease

Cited 19 time in Web of Science Cited 18 time in Scopus
Authors
Chung, Eun Joo; Choi, Sang Hoon; Shim, Yhong-Hee; Bang, Yung-Jue; Hur, Kuy Chung; Kim, Chul Woo
Issue Date
2000-08
Citation
Cellular Immunology, Vol.204 No.1, pp.46-54
Keywords
TGF-betabcl-x(L)murine T cell lineapoptosis
Abstract
Transforming growth factor-beta (TGF-beta) has been known as a potent immunosuppressive cytokine that can induce apoptosis in lymphoid cells. We established an IL-2-independent cell line, CTLL-2A, from murine T cell line CTLL-2. CTLL-2A expressed higher levels of CD95, CD69, and CD18 molecules than CTLL-2 did, suggesting a more activated state in CTLL-2A than in the CTLL-2 by phenotype. Exposing both CTLL-2 and CTLL-2A to TGF-beta results in differential apoptosis patterns defined by DNA fragmentation and plasma membrane alteration. Among the bcl-2 family members, bcl-2, bcl-w, and bcl-x(L) were also differently expressed in these two cell lines. In CTLL-2A, bcl-x(L) was amplified as a major anti-apoptotic molecule, and TGF-beta-induced cell death was more enhanced than in the original cell line. Caspase 1-like protease was activated by TGF-beta treatment and consequently it cleaved bcl-x(L) in CTLL-2A. TGF-beta-induced DNA fragmentation and cleavage of bcl-x(L), were inhibited by pretreatment with tetra peptide caspase 1 inhibitor, YVAD.cmk. These findings suggest that TGF-beta induces cell death in activated murine T cells through cleavage of bcl-x(L) via activated caspase 1-like protease, which may act as an important executor in that process. (C) 2000 Academic Press.
ISSN
0008-8749
URI
https://hdl.handle.net/10371/173038
DOI
https://doi.org/10.1006/cimm.2000.1694
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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