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A novel ets-related transcription factor, ERT/ESX/ESE-1, regulates expression of the transforming growth factor-β type II receptor

Cited 104 time in Web of Science Cited 106 time in Scopus
Authors
Choi, Shin-Geon; Yi, Youngsuk; Kim, Yong-Seok; Kato, Mariko; Chang, Jay; Chung, Hwan-Wook; Hahm, Ki-Baik; Yang, Han-Kwang; Rhee, Horace H.; Bang, Yung-Jue; Kim, Seong-Jin
Issue Date
1998-01
Citation
Journal of Biological Chemistry, Vol.273 No.1, pp.110-117
Abstract
A 2.5-kilobase cDNA clone that encodes a 371-amino acid novel transcription factor was isolated from a human placenta cDNA library using a yeast one-hybrid system, The novel ets-related transcription factor (ERT) showed a homology with the ETS DNA-binding domain, Using constructs of the transforming growth factor-beta (TGF-beta) type II receptor (RII) promoter linked to the luciferase gene, we have demonstrated that ERT activates transcription of the TGF-beta RII gene through the 5'-TTTCCTGTTTCC-3' response element spanning nucleotides +13 to +24 and multiple additional ETS binding sites between -1816 and -82 of the TGF-beta RII promoter, A specific interaction between ERT and the ETS binding sites was also demonstrated using an electrophoretic mobility shift assay, Deletion mapping of ERT protein suggests that the transactivation domain resides in the amino terminus while the DNA-binding domain is localized to the carboxyl-terminal region, Our results suggest that ERT might be a major transcription factor involved in the transcriptional regulation of the TGF-beta RII gene.
ISSN
0021-9258
URI
https://hdl.handle.net/10371/173055
DOI
https://doi.org/10.1074/jbc.273.1.110
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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