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Transcriptional inactivation of the tissue inhibitor of metalloproteinase-3 gene by DNA hypermethylation of the 5'-CpG island in human gastric cancer cell lines
DC Field | Value | Language |
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dc.contributor.author | Kang, Shin Hyeok | - |
dc.contributor.author | Choi, Hyun Ho | - |
dc.contributor.author | Kim, Sang Gyun | - |
dc.contributor.author | Jong, Hyun‐Soon | - |
dc.contributor.author | Kim, Noe Kyeong | - |
dc.contributor.author | Kim, Seong‐Jin | - |
dc.contributor.author | Bang, Yung‐Jue | - |
dc.date.accessioned | 2021-01-31T11:53:16Z | - |
dc.date.available | 2021-01-31T11:53:16Z | - |
dc.date.created | 2020-12-21 | - |
dc.date.issued | 2000-06 | - |
dc.identifier.citation | International Journal of Cancer, Vol.86 No.5, pp.632-635 | - |
dc.identifier.issn | 0020-7136 | - |
dc.identifier.other | 119282 | - |
dc.identifier.uri | https://hdl.handle.net/10371/173107 | - |
dc.description.abstract | The tissue inhibitor of metalloproteinase-3 (TIMP-3), a recently cloned member of TIMP gene family, has been implicated in the negative regulation of tumor cell invasion and tumor growth. Down-regulation of this gene has been shown to occur in a mouse carcinogenesis model, suggesting that it might play a role in the tumor progression of some cancers. In this study, we used human gastric cancer cell lines to investigate whether TIMP-3 gene expression is suppressed in human gastric cancer. We examined whether aberrant DNA methylation of the 5'-CpG island of the TIMP-3 gene is involved in this cancer. Nine of 10 human gastric cancer cell lines completely lost TIMP-3 gene expression compared with normal samples. Southern blot analysis and bisulfite genomic sequencing revealed aberrant hypermethylation near the transcription-start site of the TIMP-3 gene in all cell lines lacking TIMP-3 expression. Treatment of these cell lines with the demethylating agent 5-aza-2'-deoxycytidine restored TIMP-3 gene expression. Our results suggest that the TIMP-3 gene is another early target of tumor-associated aberrant DNA methylation in human gastric carcinogenesis. Consequently, genetic silencing of TIMP-3 may lead to a more malignant and invasive phenotype in these cancer cells. (C) 2000 Wiley-Liss. Inc. | - |
dc.language | 영어 | - |
dc.publisher | John Wiley & Sons Inc. | - |
dc.title | Transcriptional inactivation of the tissue inhibitor of metalloproteinase-3 gene by DNA hypermethylation of the 5'-CpG island in human gastric cancer cell lines | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 방영주 | - |
dc.identifier.doi | 10.1002/(SICI)1097-0215(20000601)86:5<632::AID-IJC5>3.0.CO;2-5 | - |
dc.citation.journaltitle | International Journal of Cancer | - |
dc.identifier.wosid | 000086893000005 | - |
dc.identifier.scopusid | 2-s2.0-0034059499 | - |
dc.citation.endpage | 635 | - |
dc.citation.number | 5 | - |
dc.citation.startpage | 632 | - |
dc.citation.volume | 86 | - |
dc.identifier.sci | 000086893000005 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Noe Kyeong | - |
dc.contributor.affiliatedAuthor | Bang, Yung‐Jue | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR GENE | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | METHYLATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | PROMOTER | - |
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