Browse

Mutational analysis of the hMSH2 gene in a wide variety of tumors

Cited 0 time in Web of Science Cited 8 time in Scopus
Authors
Hatta, Y; Wada, M; Takeuchi, S; Tasaka, T; Lee, E; Lee, YY; Kim, BK; Bang, Yung-Jue; Lee, S; Yamada, Y; Tomonaga, M; Wilczynski, SP; Said, JW; Koeffler, HP
Issue Date
1997-09
Citation
International Journal of Oncology, Vol.11 No.3, pp.465-469
Keywords
hMSH2DNA repair genePCR-SSCPmicrosatellite instability
Abstract
The hMSH2 gene participates in DNA mismatch repair and its mutation can result in genetic instability of the human genome which is an important feature of tumorigenesis. In this study, genetic alterations of the hMSH2 gene were examined in 43 ovarian, 36 non-small cell lung (NSCL), 31 poorly differentiated gastric, 15 endometrial, and 11 colon cancers, nine gastric cancer cell lines, 41 adult T-cell leukemias (ATLs), two ATL cell lines, and 37 non-Hodgkin's lymphomas (NHLs), using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique. Microsatellite instability (MSI) was also investigated for ovarian, NSCL, and colon cancers. The incidence of MSI was 1/36 (3%) for NSCL, 2/23 (9%) for ovarian, and 1/11 (9%) for colon cancers. Missense base changes of the hMSH2 gene were identified in two gastric cancer patients (ATG to ATA resulting in Met changing to Ile at codon 688 in exon 13 and ACA to GCA resulting in Thr changing to Ala at codon 803 in exon 14). These mutations were found in samples with no MSI. One ovarian and one gastric cancer, and six ATL samples showed two types of polymorphisms of hMSH2 (CTT to TTT resulting in Leu changing to Phe at codon 390 in exon 7 and CAG to AAG resulting in Gin to Arg at codon 419 in exon 7). Our data suggest that MSI and hMSH2 mutations are uncommon in sporadic tumors.
ISSN
1019-6439
URI
https://hdl.handle.net/10371/173119
DOI
https://doi.org/10.3892/ijo.11.3.465
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse