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TGF-β suppresses COX-2 expression by tristetraprolin-mediated RNA destabilization in A549 human lung cancer cells : TGF-beta suppresses COX-2 expression by tristetraprolin-mediated RNA destabilization in A549 human lung cancer cells
DC Field | Value | Language |
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dc.contributor.author | Kang, Soyeong | - |
dc.contributor.author | Min, Ahrum | - |
dc.contributor.author | Im, Seock-Ah | - |
dc.contributor.author | Song, Sang-Hyun | - |
dc.contributor.author | Kim, Sang Gyun | - |
dc.contributor.author | Kim, Hyun-Ah | - |
dc.contributor.author | Kim, Hee-Jun | - |
dc.contributor.author | Oh, Do-Youn | - |
dc.contributor.author | Jong, Hyun-Soon | - |
dc.contributor.author | Kim, Tae-You | - |
dc.contributor.author | Bang, Yung-Jue | - |
dc.date.accessioned | 2021-01-31T12:01:33Z | - |
dc.date.available | 2021-01-31T12:01:33Z | - |
dc.date.created | 2018-08-13 | - |
dc.date.created | 2018-08-13 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.citation | Cancer Research and Treatment, Vol.47 No.1, pp.101-109 | - |
dc.identifier.issn | 1598-2998 | - |
dc.identifier.other | 43303 | - |
dc.identifier.uri | https://hdl.handle.net/10371/173216 | - |
dc.description.abstract | Purpose Overexpression of cyclooxygenase 2 (COX-2) is thought to promote survival of transformed cells. Transforming growth factor beta (TGF-beta) exerts anti-proliferative effects on a broad range of epithelial cells. In the current study, we investigated whether TGF-beta can regulate COX-2 expression in A549 human lung adenocarcinoma cells, which are TGF-beta-responsive and overexpress COX-2. Materials and Methods Western blotting, Northern blotting, and mRNA stability assays were performed to demonstrate that COX-2 protein and mRNA expression were suppressed by TGF-beta. We also evaluated the effects of tristetraprolin (UP) on COX-2 mRNA using RNA interference. Results We demonstrated that COX-2 mRNA and protein expression were both significantly suppressed by TGF-beta. An actinomycin D chase experiment demonstrated that COX-2 mRNA was more rapidly degraded in the presence of TGF-beta, suggesting that TGF-beta-induced inhibition of COX-2 expression is achieved via decreased mRNA stability. We also found that TGF-beta rapidly and transiently induced the expression of UP, a well-known mRNA destabilizing factor, before suppression of COX-2 mRNA expression was observed. Using RNA interference, we confirmed that increased UP levels play a pivotal role in the destabilization of COX-2 mRNA by TGF-beta. Furthermore, we showed that Smad3 is essential to UP-dependent down-regulation of COX-2 expression in response to TGF-beta. Conclusion The results of this study show that TGF-beta down-regulated COX-2 expression via mRNA destabilization mediated by Smad3/TTP in A549 cells. | - |
dc.language | 영어 | - |
dc.publisher | 대한암학회 | - |
dc.title | TGF-β suppresses COX-2 expression by tristetraprolin-mediated RNA destabilization in A549 human lung cancer cells | - |
dc.title.alternative | TGF-beta suppresses COX-2 expression by tristetraprolin-mediated RNA destabilization in A549 human lung cancer cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 임석아 | - |
dc.identifier.doi | 10.4143/crt.2013.192 | - |
dc.citation.journaltitle | Cancer Research and Treatment | - |
dc.identifier.wosid | 000348095900012 | - |
dc.identifier.scopusid | 2-s2.0-84921525461 | - |
dc.citation.endpage | 109 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 101 | - |
dc.citation.volume | 47 | - |
dc.identifier.sci | 000348095900012 | - |
dc.identifier.kciid | ART001955906 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Im, Seock-Ah | - |
dc.contributor.affiliatedAuthor | Kim, Sang Gyun | - |
dc.contributor.affiliatedAuthor | Oh, Do-Youn | - |
dc.contributor.affiliatedAuthor | Kim, Tae-You | - |
dc.contributor.affiliatedAuthor | Bang, Yung-Jue | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | GROWTH-FACTOR BETA-1 | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | ADENOCARCINOMA | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordPlus | RAS | - |
dc.subject.keywordAuthor | Cyclooxygenase 2 | - |
dc.subject.keywordAuthor | Transforming growth factor beta | - |
dc.subject.keywordAuthor | Tristetraprolin | - |
dc.subject.keywordAuthor | RNA stability | - |
dc.subject.keywordAuthor | Smad3 | - |
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