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Performance of the QPLEX™ Alz plus assay, a novel multiplex kit for screening cerebral amyloid deposition

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Authors
Park, Jong-Chan; Jung, Keum Sim; Kim, Jiyeong; Jang, Ji Sung; Kwon, Sunghoon; Byun, Min Soo; Yi, Dahyun; Byeon, Gihwan; Jung, Gijung; Kim, Yu Kyeong; Lee, Dong Young; Han, Sun-Ho; Mook-Jung, Inhee
Issue Date
2021-01-06
Publisher
BMC
Citation
Alzheimer's Research & Therapy. 2021 Jan 06;13(1):12
Keywords
Alzheimer’s diseasePittsburgh compound BCerebral amyloid depositionBlood-based biomarkerQPLEX™ Alz plus assay
Abstract
Background
Alzheimers disease (AD) is an irreversible neurodegenerative disease characterized by the hallmark finding of cerebral amyloid deposition. Many researchers have tried to predict the existence of cerebral amyloid deposition by using easily accessible blood plasma samples, but the effectiveness of such strategies remains controversial.

Methods
We developed a new multiplex kit, the QPLEX™ Alz plus assay kit, which uses proteomics-based blood biomarkers to prescreen for cerebral amyloid deposition. A total of 300 participants who underwent Pittsburgh compound B (PiB)-positron emission tomography (PET) which allows imaging of cerebral amyloid deposition were included in this study. We compared the levels of QPLEX™ biomarkers between patients who were classified as PiB-negative or PiB-positive, regardless of their cognitive function. Logistic regression analysis followed by receiver operating characteristic (ROC) curve analysis was performed. The kit accuracy was tested using a randomized sample selection method.

Results
The results obtained using our assay kit reached 89.1% area under curve (AUC) with 80.0% sensitivity and 83.0% specificity. Further validation of the QPLEX™ Alz plus assay kit using a randomized sample selection method showed an average accuracy of 81.5%.

Conclusions
Our QPLEX™ Alz plus assay kit provides preliminary evidence that it can be used as blood marker to predict cerebral amyloid deposition but independent validation is needed.
ISSN
1758-9193
Language
English
URI
https://doi.org/10.1186/s13195-020-00751-x

https://hdl.handle.net/10371/173571
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Journal Papers (저널논문_의학과)
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