Isolation and Characterization of Salmonella Jumbo-Phage pSal-SNUABM-04

Abstract

The increasing emergence of antimicrobial resistance has become a global issue. Therefore,many researchers have attempted to develop alternative antibiotics. One promising alternativeis bacteriophage. In this study, we focused on a jumbo-phage infectingSalmonellaisolated fromexotic pet markets. Using aSalmonellastrain isolated from reptiles as a host, we isolated andcharacterized the novel jumbo-bacteriophage pSal-SNUABM-04. This phage was investigated interms of its morphology, host infectivity, growth and lysis kinetics, and genome. The phage wasclassified asMyoviridaebased on its morphological traits and showed a comparatively wide hostrange. The lysis efficacy test showed that the phage can inhibit bacterial growth in the planktonic state.Genetic analysis revealed that the phage possesses a 239,626-base pair genome with 280 putativeopen reading frames, 76 of which have a predicted function and 195 of which have none. By genomecomparison with other jumbo phages, the phage was designated as a novel member ofMachinaviruscomposed ofErwninaphages.