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Establishment of particulate matter-induced lung injury model in mouse
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Se Yong | - |
dc.contributor.author | An, Kyu Sup | - |
dc.contributor.author | Lee, Buhyun | - |
dc.contributor.author | Kang, Ju-Hee | - |
dc.contributor.author | Jung, Hyun Jin | - |
dc.contributor.author | Kim, Min Woo | - |
dc.contributor.author | Ryu, Hyeon Yeol | - |
dc.contributor.author | Shim, Kyu-Suk | - |
dc.contributor.author | Nam, Ki Taek | - |
dc.contributor.author | Yoon, Yeo Sung | - |
dc.contributor.author | Oh, Seung Hyun | - |
dc.date.accessioned | 2021-08-24T02:10:38Z | - |
dc.date.available | 2021-08-24T11:12:04Z | - |
dc.date.issued | 2021-07-30 | - |
dc.identifier.citation | Laboratory Animal Research. 2021 Jul 30;37(1):20 | ko_KR |
dc.identifier.issn | 2233-7660 | - |
dc.identifier.uri | https://hdl.handle.net/10371/174831 | - |
dc.description.abstract | Background
Particulate matter (PM) is one of the principal causes of human respiratory disabilities resulting from air pollution. Animal models have been applied to discover preventive and therapeutic drugs for lung diseases caused by PM. However, the induced severity of lung injury in animal models using PM varies from study to study due to disparities in the preparation of PM, and the route and number of PM administrations. In this study, we established an in vivo model to evaluate PM-induced lung injury in mice. Results PM dispersion was prepared using SRM2975. Reactive oxygen species were increased in MLE 12 cells exposed to this PM dispersion. In vivo studies were conducted in the PM single challenge model, PM multiple challenge model, and PM challenge with ovalbumin-induced asthma using the PM dispersion. No histopathological changes were observed in lung tissues after a single injection of PM, whereas mild to moderate lung inflammation was obtained in the lungs of mice exposed to PM three times. However, fibrotic changes were barely seen, even though transmission electron microscopy (TEM) studies revealed the presence of PM particles in the alveolar macrophages and alveolar capillaries. In the OVA-PM model, peribronchial inflammation and mucous hypersecretion were more severe in the OVA+PM group than the OVA group. Serum IgE levels tended to increase in OVA+PM group than in OVA group. Conclusions In this study, we established a PM-induced lung injury model to examine the lung damage induced by PM. Based on our results, repeated exposures of PM are necessary to induce lung inflammation by PM alone. PM challenge, in the presence of underlying diseases such as asthma, can also be an appropriate model for studying the health effect of PM. | ko_KR |
dc.description.sponsorship | This research was supported by Univera Co., Ltd., as one of the CAP projects and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2020R1A6A1A03043708). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Air pollution | - |
dc.subject | Particulate matter | - |
dc.subject | Animal model | - |
dc.subject | Lung injury | - |
dc.subject | Asthma | - |
dc.title | Establishment of particulate matter-induced lung injury model in mouse | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 박세용 | - |
dc.contributor.AlternativeAuthor | 안규섭 | - |
dc.contributor.AlternativeAuthor | 이부현 | - |
dc.contributor.AlternativeAuthor | 강주희 | - |
dc.contributor.AlternativeAuthor | 정현진 | - |
dc.contributor.AlternativeAuthor | 김민우 | - |
dc.contributor.AlternativeAuthor | 유현열 | - |
dc.contributor.AlternativeAuthor | 심규석 | - |
dc.contributor.AlternativeAuthor | 남기택 | - |
dc.contributor.AlternativeAuthor | 윤여성 | - |
dc.contributor.AlternativeAuthor | 오승현 | - |
dc.identifier.doi | 10.1186/s42826-021-00097-x | - |
dc.citation.journaltitle | Laboratory Animal Research | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2021-08-01T03:17:35Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 20 | ko_KR |
dc.citation.volume | 37 | ko_KR |
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