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Studies on the molecular mechanism of HOS1-mediated phytochrome B function during hypocotyl growth of Arabidopsis : 애기장대의 배축 길이 성장 과정에 있어서 HOS1을 통한 phytochrome B 기능 조절에 대한 분자적 메커니즘 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 박충모 | - |
dc.contributor.author | 김주헌 | - |
dc.date.accessioned | 2021-11-30T05:01:34Z | - |
dc.date.available | 2021-11-30T05:01:34Z | - |
dc.date.issued | 2021-02 | - |
dc.identifier.other | 000000163911 | - |
dc.identifier.uri | https://hdl.handle.net/10371/176167 | - |
dc.identifier.uri | https://dcollection.snu.ac.kr/common/orgView/000000163911 | ko_KR |
dc.description | 학위논문 (석사) -- 서울대학교 대학원 : 자연과학대학 화학부, 2021. 2. 박충모. | - |
dc.description.abstract | During seedling development, response to light induces inhibition of hypocotyl elongation, cotyledon opening, and leaf greening, and those are called photomorphogenesis. For hypocotyl elongation, photoreceptors, especially one of the red/far-red light-sensing phytochromes, phytochrome B, is regarded as a crucial regulator of it.
Although, it is not completely known how the phytochrome B regulates hypocotyl growth. Here, I showed that well known E3 ubiquitin ligase, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1, prevents the transcriptional activation activity of PHYTOCHROME INTERACTING FACTOR 4 which induces hypocotyl cell growth. Also, photoactivated phyB is needed for activation of HOS1 in inhibiting PIF4 activity. Consequently, I found a new molecular mechanism for the phyB-dependent prohibition of hypocotyl growth in Arabidopsis. | - |
dc.description.tableofcontents | INTRODUCTION 1
MATERIALS AND METHODS 3 Plant materials and growth conditions. 3 Measurement of growth kinetics 3 Analysis of gene transcript levels 3 Yeast two hybrid assays. 4 BiFC assay. 4 ChIP assay. 4 Transcriptional activation activity assays. 5 Coimmunoprecipitation assay. 5 Cell fractionation assay. 6 RESULTS 8 HOS1 is associated with PIF4 function during hypocotyl elongation 8 HOS1 inhibits the transcriptional activation activity of PIF4 12 HOS1 is required for the phyB-mediated inhibition of PIF4 function 17 phyB activates HOS1 in inhibiting PIF4 activity 19 DISCUSSION 25 HOS1 is required for phyB-mediated inhibition of PIF4 activity 25 Requirement of additional factors for HOS1-mediated regulation of PIF4 26 How does phyB activates HOS1? 26 ACKNOWLEDGEMENT 28 REFERENCES 29 ABSTRACT IN KOREAN 34 | - |
dc.format.extent | ix, 35 | - |
dc.language.iso | eng | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | hypocotyl growth | - |
dc.subject | phytochrome signaling | - |
dc.subject | HOS1 | - |
dc.subject | PIF4 | - |
dc.subject | phyB | - |
dc.subject | 배축 길이 성장 | - |
dc.subject | 파이토크롬 | - |
dc.subject | 광형태형성 | - |
dc.subject.ddc | 540 | - |
dc.title | Studies on the molecular mechanism of HOS1-mediated phytochrome B function during hypocotyl growth of Arabidopsis | - |
dc.title.alternative | 애기장대의 배축 길이 성장 과정에 있어서 HOS1을 통한 phytochrome B 기능 조절에 대한 분자적 메커니즘 연구 | - |
dc.type | Thesis | - |
dc.type | Dissertation | - |
dc.contributor.AlternativeAuthor | Kim Ju-Heon | - |
dc.contributor.department | 자연과학대학 화학부 | - |
dc.description.degree | Master | - |
dc.date.awarded | 2021-02 | - |
dc.contributor.major | 생화학 | - |
dc.identifier.uci | I804:11032-000000163911 | - |
dc.identifier.holdings | 000000000044▲000000000050▲000000163911▲ | - |
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