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Comparison of sampling methods in assessing the microbiome from patients with ulcerative colitis
DC Field | Value | Language |
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dc.contributor.author | Kim, Dan | - |
dc.contributor.author | Jung, Jun-Young | - |
dc.contributor.author | Oh, Hyun-Seok | - |
dc.contributor.author | Jee, Sam-Ryong | - |
dc.contributor.author | Park, Sung Jae | - |
dc.contributor.author | Lee, Sang-Heon | - |
dc.contributor.author | Yoon, Jun-Sik | - |
dc.contributor.author | Yu, Seung Jung | - |
dc.contributor.author | Yoon, In-Cheol | - |
dc.contributor.author | Lee, Hong Sub | - |
dc.date.accessioned | 2022-02-14T06:36:34Z | - |
dc.date.available | 2022-02-14T15:37:40Z | - |
dc.date.issued | 2021-10-22 | - |
dc.identifier.citation | BMC Gastroenterology. 2021 Oct 22;21(1):396 | ko_KR |
dc.identifier.issn | 1471-230X | - |
dc.identifier.uri | https://hdl.handle.net/10371/176950 | - |
dc.description.abstract | Background
Dysbiosis of ulcerative colitis (UC) has been frequently investigated using readily accessible stool samples. However, stool samples might insufficiently represent the mucosa-associated microbiome status. We hypothesized that luminal contents including loosely adherent luminal bacteria after bowel preparation may be suitable for diagnosing the dysbiosis of UC. Methods This study included 16 patients with UC (9 men and 7 women, mean age: 52.13 ± 14.09years) and 15 sex- and age-matched healthy individuals (8 men and 7 women, mean age: 50.93 ± 14.11years). They donated stool samples before colonoscopy and underwent luminal content aspiration and endoscopic biopsy during the colonoscopy. Then, the composition of each microbiome sample was analyzed by 16S rRNA-based next-generation sequencing. Results The microbiome between stool, luminal contents, and biopsy was significantly different in alpha and beta diversities. However, a correlation existed between stool and luminal contents in the Procrustes test (p = 0.001) and Mantel test (p = 0.0001). The stool microbiome was different between patients with UC and the healthy controls. Conversely, no difference was found in the microbiome of luminal content and biopsy samples between the two subject groups. The microbiome of stool and lavage predicted UC, with AUC values of 0.85 and 0.81, respectively. Conclusion The microbiome of stool, luminal contents, and biopsy was significantly different. However, the microbiome of luminal contents during colonoscopy can predict UC, with AUC values of 0.81. Colonoscopic luminal content aspiration analysis could determine microbiome differences between patients with UC and the healthy control, thereby beneficial in screening dysbiosis via endoscopy. Trial registration: This trial was registered at http://cris.nih.go.kr . Registration No.: KCT0003352), Date: 2018–11-13. | ko_KR |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) (No. 2020R1G1A100923411). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Colon lavage | - |
dc.subject | Colonoscopy | - |
dc.subject | Infammatory bowel disease | - |
dc.subject | Microbiome | - |
dc.subject | Ulcerative colitis | - |
dc.title | Comparison of sampling methods in assessing the microbiome from patients with ulcerative colitis | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김단 | - |
dc.contributor.AlternativeAuthor | 정준영 | - |
dc.contributor.AlternativeAuthor | 오현석 | - |
dc.contributor.AlternativeAuthor | 이삼룡 | - |
dc.contributor.AlternativeAuthor | 박성재 | - |
dc.contributor.AlternativeAuthor | 이상헌 | - |
dc.contributor.AlternativeAuthor | 윤준식 | - |
dc.contributor.AlternativeAuthor | 윤인철 | - |
dc.contributor.AlternativeAuthor | 이홍섭 | - |
dc.identifier.doi | https://doi.org/10.1186/s12876-021-01975-3 | - |
dc.citation.journaltitle | BMC Gastroenterology | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2021-10-24T03:12:19Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 396 | ko_KR |
dc.citation.volume | 21 | ko_KR |
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