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ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8

Cited 23 time in Web of Science Cited 23 time in Scopus

Kwon, S Chul; Jang, Harim; Shen, Siyuan; Baek, S Chan; Kim, Kijun; Yang, Jihye; Kim, Jeesoo; Kim, Jong-Seo; Wang, Suman; Shi, Yunyu; Li, Fudong; Kim, V Narry

Issue Date
Oxford University Press
Nucleic Acids Research, Vol.48 No.19, pp.11097-11112
The microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a new component of Microprocessor. Through a crystal structure and biochemical experiments, we reveal that ERH uses its hydrophobic groove to bind to a conserved region in the N-terminus of DGCR8, in a 2:2 stoichiometry. Knock-down of ERH or deletion of the DGCR8 N-terminus results in a reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases the processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144. Thus, the ERH dimer may mediate 'cluster assistance' in which Microprocessor is loaded onto a poor substrate with help from a high-affinity substrate in the same cluster. Our study reveals a role of ERH in the miRNA biogenesis pathway.
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  • College of Natural Sciences
  • School of Biological Sciences
Research Area Molecular Interactomics, Proteomics, Systems Biology, 단백체학, 분자상호작용체학, 시스템생물학


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