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Studies on the supernumerary centrioles in the breast cancer cell lines : 유방암 세포주에서 중심립 숫자 조절에 관한 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이건수 | - |
dc.contributor.author | 이예주 | - |
dc.date.accessioned | 2022-04-20T07:55:16Z | - |
dc.date.available | 2022-04-20T07:55:16Z | - |
dc.date.issued | 2021 | - |
dc.identifier.other | 000000166823 | - |
dc.identifier.uri | https://hdl.handle.net/10371/178999 | - |
dc.identifier.uri | https://dcollection.snu.ac.kr/common/orgView/000000166823 | ko_KR |
dc.description | 학위논문(석사) -- 서울대학교대학원 : 자연과학대학 생명과학부, 2021.8. 이건수. | - |
dc.description.abstract | Centrosomes are major microtubule organizer in animal cells. It is critical to maintain two centrosomes in a single cell since they function as spindle poles to pull a set of chromosomes into two daughter cells. However, deregulations in centrosome numbers are frequently observed in cancer cells. Here, I investigated generation of the supernumerary centrioles in selected breast cancer cells. The number of centrioles at specific stages of the cell cycle was determined with the coimmunostaining analyses using the BrdU and centrin-2 antibodies. The results showed that supernumerary centrioles are maintained at specific subset of the breast cancer cell populations. The proportions of the cells with supernumerary centrioles were not identical during the cell cycle. In case of MDA-MB-231 cells, proportion of the cells with supernumerary centrioles were maintained to 20% throughout the cell cycle. However, in case of Hs578t cells, supernumerary centrioles were detected in 5% of the S phase cells, but in 50% of the M phase cells. I also detected premature separation of centrioles in Hs578t cells at mitosis. These results suggest that supernumerary centrioles may be generated at specific cell cycle stages, possibly following distinct amplifying mechanisms. The breast cancer cell lines may adopt their own mechanisms for amplifying their centrioles. | - |
dc.description.tableofcontents | INTRODUCTION 1
MATERIALS AND METHODS 6 Cell culture, Cell synchronization and Drug treatments 6 Antibodies 7 Immunostaining analysis 7 Fluorescence-activated cell sorting 9 RESULTS 10 Supernumerary centrioles in the breast cancer cells 10 Generation of supernumerary centrioles at M phase 13 Precocious centriole separation of Hs578t cells at M phase .17 DISCUSSION 26 REFERENCES 30 ABSTRACT IN KOREAN 33 LIST OF FigureS . Figure 1. Supernumerary centrioles in the breast cancer cells 18 Figure 2. Determination of the cell cycle stages of individual cells 19 Figure 3. Determination of the number of centrioles at cell cycle stages of the breast cancer cells 20 Figure 4. Determination of the number of centrioles in the breast cancer cells after the treatment of the cell cycle drugs 21 Figure 5. Determination of the number of centrioles in synchronized populations of the breast cancer cells 22 Figure 6. Premature centriole separation at M phase of the breast cancer cells 23 Table 1. The statistical number and percentage of CETN2 dots discovered in cell cycle stages in asynchronous and drug treated breast cancer cell lines 24 Table 2. The statistical number and percentage of CETN2 dots discovered in cell cycle stages after the double thymidine block and release . 25 | - |
dc.format.extent | v, 34 | - |
dc.language.iso | eng | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Supernumerary centrioles | - |
dc.subject | Breast cancer cell line | - |
dc.subject | PLK4 | - |
dc.subject | PCNT | - |
dc.subject | CEP215 | - |
dc.subject | Interphase | - |
dc.subject | Mitosis | - |
dc.subject.ddc | 570 | - |
dc.title | Studies on the supernumerary centrioles in the breast cancer cell lines | - |
dc.title.alternative | 유방암 세포주에서 중심립 숫자 조절에 관한 연구 | - |
dc.type | Thesis | - |
dc.type | Dissertation | - |
dc.contributor.AlternativeAuthor | Yejoo Lee | - |
dc.contributor.department | 자연과학대학 생명과학부 | - |
dc.description.degree | 석사 | - |
dc.date.awarded | 2021-08 | - |
dc.contributor.major | 조직 세포학 | - |
dc.identifier.uci | I804:11032-000000166823 | - |
dc.identifier.holdings | 000000000046▲000000000053▲000000166823▲ | - |
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