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DARPP-32의 인간 신경줄기세포 증식과 생존에서의 역할 규명 : Role of DARPP-32 in the Proliferation and Survival of Human Neural Stem Cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Juhee | - |
dc.contributor.author | Chang, Mi Sook | - |
dc.date.accessioned | 2022-05-16T06:44:47Z | - |
dc.date.available | 2022-05-16T06:44:47Z | - |
dc.date.created | 2021-02-26 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.citation | 대한구강해부학회지, Vol.41 No.1, pp.97-108 | - |
dc.identifier.issn | 1225-6803 | - |
dc.identifier.uri | https://hdl.handle.net/10371/179719 | - |
dc.description.abstract | To investigate the role of dopamine- and cAMP-regulated neuronal phosphoprotein 32 kDa (DARPP-32), also known as protein phosphatase 1 regulatory subunit 1B (PPP1R1B), in cell proliferation and survival of human neural stem cells (hiNSCs) induced from human adipose-derived stem cells, DARPP-32 expression was knocked down by a lentiviral vector. Cell proliferation was relatively slower and doubling time was longer in the DARPP-32-knockdown hNSCs than in the scrambled control. Oxidative stress testing showed that DARPP-32 knockdown caused more cell death in hiNSCs after hydrogen peroxide (H2O2) treatment than in the scrambled control. The expressions of CTNNB1, CXCR4, and VCAM-1, which are related to cell proliferation, were lower in the DARPP-32-knockdown hiNSCs than in the scrambled control. In addition, DARPP-32 knockdown decreased the expression of Bcl-2 and increased the expression of Bax in response to H2O2 treatment.
We also confirmed reduced expression of phosphorylated Akt (p-AKT) in DARPP-32-knockdown hiNSCs. In conclusion, these findings demonstrate the mechanisms by which DARPP-32 positively affects cell proliferation and survival in hiNSCs. | - |
dc.language | 영어 | - |
dc.publisher | 대한구강해부학회 | - |
dc.title | DARPP-32의 인간 신경줄기세포 증식과 생존에서의 역할 규명 | - |
dc.title.alternative | Role of DARPP-32 in the Proliferation and Survival of Human Neural Stem Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.35607/kjoa.41.1.202012.009 | - |
dc.citation.journaltitle | 대한구강해부학회지 | - |
dc.citation.endpage | 108 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 97 | - |
dc.citation.volume | 41 | - |
dc.identifier.kciid | ART002670671 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Chang, Mi Sook | - |
dc.type.docType | Article | - |
dc.description.journalClass | 2 | - |
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