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Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization
DC Field | Value | Language |
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dc.contributor.author | Jang, Hee-Won | - |
dc.contributor.author | An, Ju-Hyun | - |
dc.contributor.author | Kim, Kyeong Bo | - |
dc.contributor.author | Lee, Jeong-Hwa | - |
dc.contributor.author | Oh, Ye-In | - |
dc.contributor.author | Park, Su-Min | - |
dc.contributor.author | Chae, Hyung-Kyu | - |
dc.contributor.author | Youn, Hwa-Young | - |
dc.date.accessioned | 2022-05-16T08:51:59Z | - |
dc.date.available | 2022-05-16T08:51:59Z | - |
dc.date.created | 2022-03-07 | - |
dc.date.created | 2022-03-07 | - |
dc.date.issued | 2021-11 | - |
dc.identifier.citation | PLoS ONE, Vol.16 No.11 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://hdl.handle.net/10371/179790 | - |
dc.description.abstract | Background Preconditioning with lipopolysaccharide (LPS) is used to improve the secretion of anti-inflammatory agents in B cells. However, there are only a few studies on canine B cells. Objective This study aimed to evaluate the immune regulatory capacity of canine peripheral blood mononuclear cell-derived B cells pretreated with LPS. Methods Canine B cells were isolated from canine peripheral blood mononuclear cells, which were obtained from three healthy canine donors. The B cells were preconditioned with LPS, and then cell viability and the expression of the regulatory B cell marker were assessed. Finally, RNA extraction and immunofluorescence analysis were performed. Results LPS primed B cells expressed the interleukin (IL)-10 surface marker and immunoregulatory gene expression, such as IL-10, programmed death-ligand 1, and transforming growth factor beta. Macrophages in the inflammatory condition cocultured with primed B cells were found to have significantly down-regulated pro-inflammatory cytokine, such as tumor necrosis factor-alpha, and up-regulated anti-inflammatory cytokines such as IL-10. Additionally, it was revealed that co-culture with primed B cells re-polarized M1 macrophages to M2 macrophages. Conclusions This study revealed that LPS-primed B cells have an anti-inflammatory effect and can repolarize macrophages, suggesting the possibility of using LPS-primed B cells as a therapeutic agent for its anti-inflammatory effects and immune modulation. | - |
dc.language | 영어 | - |
dc.publisher | Public Library of Science | - |
dc.title | Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization | - |
dc.type | Article | - |
dc.identifier.doi | 10.1371/journal.pone.0256651 | - |
dc.citation.journaltitle | PLoS ONE | - |
dc.identifier.wosid | 000755579000002 | - |
dc.identifier.scopusid | 2-s2.0-85119987939 | - |
dc.citation.number | 11 | - |
dc.citation.volume | 16 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Youn, Hwa-Young | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | T-CELLS | - |
dc.subject.keywordPlus | IL-10 | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordPlus | MODEL | - |
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