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Lipid metabolism, immune and apoptosis transcriptomic responses of the hepatopancreas of Chinese mitten crab to the exposure to microcystin-LR

Cited 6 time in Web of Science Cited 6 time in Scopus
Authors

Chi, Cheng; Giri, Sib Sankar; Yu, Xia Wei; Liu, Yuan; Chen, Ke Ke; Liu, Wen Bin; Zhang, Ding Dong; Jiang, Guang Zhen; Li, Xiang Fei; Gao, Xin; Chen, Bin Lin; Park, Se Chang

Issue Date
2022-05
Publisher
Academic Press
Citation
Ecotoxicology and Environmental Safety, Vol.236, p. 113439
Abstract
© 2022 The AuthorsGlobal warming is favouring the incidence, intensity and duration of harmful cyanobacterial blooms. Microcystin-LR (MC-LR), a hepatotoxic agent, is produced during cyanobacterial blooms. To understand the molecular mechanisms of acute hepatotoxic effect of low doses of MC-LR in crab, we examined differentially expressed genes in samples of the hepatopancreas of Chinese mitten crab (Eriocheir sinensis) collected in 48 h after injections of MC-LR at doses of 0, 25, 50, and 75 µg/kg. The results revealed that MC-LR induced changes in corresponding gene led to the accumulation of triglycerides. MC-LR exposure affected sterol metabolism. Apoptosis-related genes such as Fas-L, Bcl-XL, Cytc, AiF, p53, PERK, calpain, CASP2, CASP7, α-tubulin, PARP, GF, G12, and PKC were upregulated. Conversely, expression levels of CASP10 and ASK1 were downregulated. Genes related to the regulation of actin cytoskeleton (Rho, ROCK, MLCP, MLC, PAK, and PFN) were upregulated. Further, expression levels of genes encoding fatty acid elongation-related enzymes were upregulated, but the expression of genes related to fatty acid synthesis was slightly down regulated. Taken together, these results demonstrated the hepatic toxicity and molecular mechanisms of changes in lipid metabolism, immune and apoptosis in Chinese mitten crab under the MC-LR-induced stress, which is the first report on crabs and performs a comprehensive analysis and a new insight of the molecular toxicological responses in crabs.
ISSN
0147-6513
URI
https://hdl.handle.net/10371/179791
DOI
https://doi.org/10.1016/j.ecoenv.2022.113439
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  • College of Veterinary Medicine
  • Department of Veterinary Medicine
Research Area Bacteriophage Therapy, Veterinary Medicine, Veterinary Microbiology

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