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Protocol A flow-cytometry-based assessment of global protein synthesis in human senescent cells : A flow-cytometry-based assessment of global protein synthesis in human senescent cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Yeonghyeon | - |
dc.contributor.author | Kim, Jaejin | - |
dc.contributor.author | Jeon, Taerang | - |
dc.contributor.author | Roh, Kyeonghwan | - |
dc.contributor.author | Kim, Mi-Sung | - |
dc.contributor.author | Kang, Chan Hee | - |
dc.date.accessioned | 2022-05-20T07:35:31Z | - |
dc.date.available | 2022-05-20T07:35:31Z | - |
dc.date.created | 2022-02-07 | - |
dc.date.created | 2022-02-07 | - |
dc.date.created | 2022-02-07 | - |
dc.date.created | 2022-02-07 | - |
dc.date.created | 2022-02-07 | - |
dc.date.created | 2022-02-07 | - |
dc.date.issued | 2021-09 | - |
dc.identifier.citation | STAR Protocols, Vol.2 No.3, p. 100809 | - |
dc.identifier.issn | 2666-1667 | - |
dc.identifier.uri | https://hdl.handle.net/10371/179965 | - |
dc.description.abstract | Senescent cells constantly experience stressful conditions and restrain their protein translation to cope with it. Here, we present a detailed protocol to measure the rate of global protein synthesis using L-azidohomoalanine (L-AHA)-based click chemistry in human senescent fibroblasts. We optimized several aspects of the procedure, including senescence induction, a flow cytometry analysis of senescent cells, and the duration of L-AHA incorporation. This protocol uses senescent human fibroblasts but can be applied to other types of cells or circumstances. For complete details on the use and execution of this protocol, please refer to Lee et al. (2021). | - |
dc.language | 영어 | - |
dc.publisher | Elsevier | - |
dc.title | Protocol A flow-cytometry-based assessment of global protein synthesis in human senescent cells | - |
dc.title.alternative | A flow-cytometry-based assessment of global protein synthesis in human senescent cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.xpro.2021.100809 | - |
dc.citation.journaltitle | STAR Protocols | - |
dc.identifier.wosid | 001048876800003 | - |
dc.identifier.scopusid | 2-s2.0-85122818488 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 100809 | - |
dc.citation.volume | 2 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kang, Chan Hee | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CELLULAR SENESCENCE | - |
dc.subject.keywordPlus | AUTOPHAGY | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordAuthor | Cell Biology | - |
dc.subject.keywordAuthor | Cell-based Assays | - |
dc.subject.keywordAuthor | Flow Cytometry/Mass Cytometry | - |
dc.subject.keywordAuthor | Molecular Biology | - |
dc.subject.keywordAuthor | Molecular/Chemical Probes | - |
dc.subject.keywordAuthor | Protein Biochemistry | - |
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