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Role of Human Primary Renal Fibroblast in TGF-beta 1-Mediated Fibrosis-Mimicking Devices : Role of human primary renal fibroblast in tgf-β1-mediated fibrosis-mimicking devices
DC Field | Value | Language |
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dc.contributor.author | Hwang, Seong-Hye | - |
dc.contributor.author | Lee, Yun-Mi | - |
dc.contributor.author | Choi, Yunyeong | - |
dc.contributor.author | Son, Hyung Eun | - |
dc.contributor.author | Ryu, Ji Young | - |
dc.contributor.author | Na, Ki Young | - |
dc.contributor.author | Chin, Ho Jun | - |
dc.contributor.author | Jeon, Noo Li | - |
dc.contributor.author | Kim, Sejoong | - |
dc.date.accessioned | 2022-05-20T07:36:17Z | - |
dc.date.available | 2022-05-20T07:36:17Z | - |
dc.date.created | 2021-11-05 | - |
dc.date.issued | 2021-10 | - |
dc.identifier.citation | International Journal of Molecular Sciences, Vol.22 No.19, p. 10758 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://hdl.handle.net/10371/179995 | - |
dc.description.abstract | Renal fibrosis is a progressive chronic kidney disease that ultimately leads to end-stage renal failure. Despite several approaches to combat renal fibrosis, an experimental model to evaluate currently available drugs is not ideal. We developed fibrosis-mimicking models using three-dimensional (3D) co-culture devices designed with three separate layers of tubule interstitium, namely, epithelial, fibroblastic, and endothelial layers. We introduced human renal proximal tubular epithelial cells (HK-2), human umbilical-vein endothelial cells, and patient-derived renal fibroblasts, and evaluated the effects of transforming growth factor-beta (TGF-beta) and TGF-beta inhibitor treatment on this renal fibrosis model. The expression of the fibrosis marker alpha smooth muscle actin upon TGF-beta 1 treatment was augmented in monolayer-cultured HK-2 cells in a 3D disease model. In the vascular compartment of renal fibrosis models, the density of vessels was increased and decreased in the TGF-beta-treated group and TGF-beta-inhibitor treatment group, respectively. Multiplex ELISA using supernatants in the TGF-beta-stimulating 3D models showed that pro-inflammatory cytokine and growth factor levels including interleukin-1 beta, tumor necrosis factor alpha, basic fibroblast growth factor, and TGF-beta 1, TGF-beta 2, and TGF-beta 3 were increased, which mimicked the fibrotic microenvironments of human kidneys. This study may enable the construction of a human renal fibrosis-mimicking device model beyond traditional culture experiments. | - |
dc.language | 영어 | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Role of Human Primary Renal Fibroblast in TGF-beta 1-Mediated Fibrosis-Mimicking Devices | - |
dc.title.alternative | Role of human primary renal fibroblast in tgf-β1-mediated fibrosis-mimicking devices | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/ijms221910758 | - |
dc.citation.journaltitle | International Journal of Molecular Sciences | - |
dc.identifier.wosid | 000707937300001 | - |
dc.identifier.scopusid | 2-s2.0-85116236041 | - |
dc.citation.number | 19 | - |
dc.citation.startpage | 10758 | - |
dc.citation.volume | 22 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Na, Ki Young | - |
dc.contributor.affiliatedAuthor | Chin, Ho Jun | - |
dc.contributor.affiliatedAuthor | Jeon, Noo Li | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
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