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Characterization of the antioxidant and anti-inflammatory properties of a polysaccharide-based bioflocculant from Bacillus subtilis F9
DC Field | Value | Language |
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dc.contributor.author | Giri, Sib Sankar | - |
dc.contributor.author | Ryu, EunChae | - |
dc.contributor.author | Park, Se Chang | - |
dc.date.accessioned | 2022-06-21T08:04:10Z | - |
dc.date.available | 2022-06-21T08:04:10Z | - |
dc.date.created | 2019-12-30 | - |
dc.date.created | 2019-12-30 | - |
dc.date.created | 2019-12-30 | - |
dc.date.created | 2019-12-30 | - |
dc.date.created | 2019-12-30 | - |
dc.date.issued | 2019-11 | - |
dc.identifier.citation | Microbial Pathogenesis, Vol.136, p. 103642 | - |
dc.identifier.issn | 0882-4010 | - |
dc.identifier.uri | https://hdl.handle.net/10371/182650 | - |
dc.description.abstract | Microbial flocculants are versatile class of novel biomacromolecules with numerous potential industrial applications. This study sought to investigate the antimicrobial, antioxidant, and anti-inflammatory potential of a polysaccharide-based bioflocculant (PBB) extracted from Bacillus subtilis F9. To achieve this, the antioxidant activity of different PBB concentrations(100 mu g/mL- 1000 mu g/mL) was first examined in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radicals, and superoxide radical scavenging assays. Further, the anti-inflammatory activity of PBB against lipopolysaccharide (LPS; 1 mu g/mL)-induced inflammatory mediators released from headkidney (HK)-derived macrophages of Labeo rohita was investigated. Our results revealed that the capacities of 800 mu g/mL of PBB to scavenge DPPH, hydroxyl radicals, and superoxide radicals were 81.46 +/- 1.37%, 66.34 +/- 2.63%, and 78.03 +/- 2.46%, respectively, which were slightly higher that observed following treatment with 400 mu g/mL of the positive control (ascorbic acid). Further, the radical scavenging capacity of PBB was found to steadily increase with increasing concentrations of PBS. Pre-treatment with PBB also inhibited nitric oxide production in a dose-dependent manner. We next examined the effect of PBB on proinflammatory cytokines (TNF-alpha, and IL-1 beta) and anti-inflammatory cytokines (IL-10, TGF-beta) via qRT-PCR and ELISA. We found that PBB markedly inhibited the LPS-induced mRNA and protein expression levels of TNF-alpha and IL-10, while it significantly increased those of IL-10 and TGF-beta. Further, PBB exhibited an antibacterial activity against multiple food-borne pathogens with minimal inhibitory concentration values in the range of 3-11 mg/mL. Importantly, PBB exhibited negligible cytotoxic effects against HK macrophages. Taken together these results suggest that PBB may serve as a natural antioxidant for application in functional therapies and may also be exploited for its anti-inflammatory potential. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Characterization of the antioxidant and anti-inflammatory properties of a polysaccharide-based bioflocculant from Bacillus subtilis F9 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.micpath.2019.103642 | - |
dc.citation.journaltitle | Microbial Pathogenesis | - |
dc.identifier.wosid | 000498333600006 | - |
dc.identifier.scopusid | 2-s2.0-85070506126 | - |
dc.citation.startpage | 103642 | - |
dc.citation.volume | 136 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Se Chang | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | INFLAMMATORY RESPONSES | - |
dc.subject.keywordPlus | EXOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordAuthor | Antibacterial | - |
dc.subject.keywordAuthor | Antioxidant | - |
dc.subject.keywordAuthor | Polysaccharide-based bioflocculant | - |
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