Inhibitory effect of D-arabinose on oral bacteria biofilm formation on titanium discs

Abstract

© 2022 Elsevier LtdObjectives: Biofilm formation on dental implant surfaces can cause peri-implant mucositis and peri-implantitis. Lectins are involved in interactions between bacteria or between bacteria and their hosts. Disrupting these interactions via specific sugars can result in reduced adhesion and biofilm formation. The purpose of this study was to identify sugars that function as antiadhesion or antibiofilm agents on titanium discs. Methods: Of the sugars tested, the sugars that did not affect the planktonic growth of Streptococcus oralis, Fusobacterium nucleatum, and Porphyromonas gingivalis were selected. The selected sugars were assessed for their ability to inhibit biofilm formation of bacteria in single and consortium species by crystal violet staining, confocal laser scanning microscopy after live/dead staining, and scanning electron microscopy. The sugars were evaluated for their ability to inhibit activity of the quorum sensing molecule autoinducer 2 (AI-2) by bioluminescence assay. Results: Biofilm formation of single bacteria or consortia of S. oralis, F. nucleatum, and P. gingivalis on titanium discs was significantly inhibited in the presence of D-arabinose. Pretreating titanium discs with D-arabinose for 3 min inhibited biofilm formation at a level comparable to that observed when D-arabinose was present over the entire period, suggesting that D-arabinose had initial anti-adhesive activity. In addition, D-arabinose inhibited the activity of AI-2. Conclusions: D-Arabinose may be a good candidate for application as an antibiofilm agent and AI-2 inhibitor.